Multilocus sequence analysis of ‘Candidatus Phytoplasma asteris’ strain and the genome analysis of Turnip mosaic virus co-infecting oilseed rape
Article first published online: 26 JUN 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 3, pages 774–785, September 2014
How to Cite
Šeruga Musić, M., Duc Nguyen, H., Černi, S., Mamula, Đ., Ohshima, K. and Škorić, D. (2014), Multilocus sequence analysis of ‘Candidatus Phytoplasma asteris’ strain and the genome analysis of Turnip mosaic virus co-infecting oilseed rape. Journal of Applied Microbiology, 117: 774–785. doi: 10.1111/jam.12567
- Issue published online: 18 AUG 2014
- Article first published online: 26 JUN 2014
- Accepted manuscript online: 10 JUN 2014 07:36AM EST
- Manuscript Accepted: 2 JUN 2014
- Manuscript Revised: 21 MAY 2014
- Manuscript Received: 7 APR 2014
- Croatian Ministry of Science, Education and Sports. Grant Number: 119-1191192-1222
- Saga University
- amp ;
- groEL ;
- rp gene;
- secY ;
- tufB ;
- TuMV genome
Molecular characterization of a pathogenic complex infecting winter oilseed rape (Brassica napus ssp. oleifera (DC.) Metzg.) plants showing typical rape phyllody symptoms along with some atypical changes.
Methods and Results
Phytoplasma (‘Candidatus Phytoplasma’) presence was confirmed by PCR-RFLP and 16S rRNA gene sequencing. Phylogenetic analyses of phytoplasma amp, tufB, secY, groEL and ribosomal protein genes confirmed its affiliation to the ‘Ca. P. asteris’ species. However, in the amp gene encoding a specific protein crucial for insect transmission specificity, significant SNPs were found. Biological and serological tests revealed the co-infection with Turnip mosaic virus (TuMV). The phylogenetic analysis of full TuMV genome sequence, the first reported from the Balkans, classified it into the world-B phylogenetic lineage.
A pathogenic complex consisting of ‘Ca. P. asteris’ and TuMV found to co-infect oilseed rape plants for the first time was molecularly characterized.
Significance and Impact of the Study
Rape phyllody is a serious problem in rapeseed production. The molecular information from this first multi-gene analysis of ‘Ca. P. asteris’ strain associated with rape phyllody as well as the first report of the complete sequence of TuMV isolate from the Balkans is a starting point for understanding the disease complexity and management.