Chitosan–protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials
Article first published online: 30 JUN 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 3, pages 634–642, September 2014
How to Cite
Szweda, P., Gorczyca, G., Tylingo, R., Kurlenda, J., Kwiecinski, J. and Milewski, S. (2014), Chitosan–protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials. Journal of Applied Microbiology, 117: 634–642. doi: 10.1111/jam.12568
- Issue published online: 18 AUG 2014
- Article first published online: 30 JUN 2014
- Accepted manuscript online: 10 JUN 2014 07:36AM EST
- Manuscript Accepted: 3 JUN 2014
- Manuscript Revised: 15 MAY 2014
- Manuscript Received: 4 MAR 2014
- Polish Ministry of Science and Higher Education. Grant Numbers: N N405 420739, N N312 258540
- Foundation for Polish Science
- European Regional Development Fund. Grant Number: VENTURES/2010-5/2
- carbon dioxide;
- wound dressing
The development of technology for preparing chitosan–protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus.
Methods and Results
The unique technology of chitosan solubilization using gaseous CO2 instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal activity against staphylococci, within the structure of chitosan–protein sponges. The developed chitosan–protein scaffolds loaded with lysostaphin revealed high antistaphylococcal activity, which has been confirmed with a large (n = 143) collection of clinical (skin and wound infections) and animal (bovine mastitis) isolates of these bacteria, including MRSA. No change of bactericidal activity of the lyophilized materials has been observed during half-year storage at 4°C.
The developed materials are potential candidates for preparing biologically active, antistaphylococcal wound dressing materials.
Significance and Impact of the Study
Staphylococci belong to the most popular and most burdensome aetiological factors of wound and soft tissues infections. The developed chitosan-protein scaffolds loaded with lysostaphin could be a possible solution to problems associated with treatment of these infections.