Chitosan–protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials

Authors

  • P. Szweda,

    Corresponding author
    1. Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdańsk, Poland
    • Correspondence

      Piotr Szweda, Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Narutowicza Str. 11/12, 80-233 Gdańsk, Poland.

      E-mail: piotr.szweda@wp.pl

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  • G. Gorczyca,

    1. Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdańsk, Poland
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  • R. Tylingo,

    1. Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdansk University of Technology, Gdańsk, Poland
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  • J. Kurlenda,

    1. State Higher Vocational School in Koszalin, Koszalin, Poland
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  • J. Kwiecinski,

    1. Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden
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  • S. Milewski

    1. Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdańsk, Poland
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Abstract

Aims

The development of technology for preparing chitosan–protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus.

Methods and Results

The unique technology of chitosan solubilization using gaseous CO2 instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal activity against staphylococci, within the structure of chitosan–protein sponges. The developed chitosan–protein scaffolds loaded with lysostaphin revealed high antistaphylococcal activity, which has been confirmed with a large (n = 143) collection of clinical (skin and wound infections) and animal (bovine mastitis) isolates of these bacteria, including MRSA. No change of bactericidal activity of the lyophilized materials has been observed during half-year storage at 4°C.

Conclusions

The developed materials are potential candidates for preparing biologically active, antistaphylococcal wound dressing materials.

Significance and Impact of the Study

Staphylococci belong to the most popular and most burdensome aetiological factors of wound and soft tissues infections. The developed chitosan-protein scaffolds loaded with lysostaphin could be a possible solution to problems associated with treatment of these infections.

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