Overexpression of erg1 gene in Trichoderma harzianum CECT 2413: effect on the induction of tomato defence-related genes
Version of Record online: 1 JUL 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 3, pages 812–823, September 2014
How to Cite
Cardoza, R.E., Malmierca, M.G. and Gutiérrez, S. (2014), Overexpression of erg1 gene in Trichoderma harzianum CECT 2413: effect on the induction of tomato defence-related genes. Journal of Applied Microbiology, 117: 812–823. doi: 10.1111/jam.12574
- Issue online: 18 AUG 2014
- Version of Record online: 1 JUL 2014
- Accepted manuscript online: 12 JUN 2014 09:33AM EST
- Manuscript Accepted: 5 JUN 2014
- Manuscript Revised: 20 MAY 2014
- Manuscript Received: 18 FEB 2014
- MINECO. Grant Numbers: AGL2009-13431-C02, AGL2012-40041-C02-02
- Junta de Castilla y León. Grant Number: LE125A12-2
- Spanish Ministry of Science and Innovation. Grant Number: AP2007-02835
Table S1 Oligonucleotides used to amplify an internal probe of erg1 gene for Southern Blot experiment.
Table S2 Parameters measured from tomato plants inoculated with the Trichoderma strains used in the present work in comparison with control plants. The diameter of lesions caused by B. cinerea 05·10 were also measured.
Figure S1 Calibration curves in which the Ct of the tomato α-actin (left panel) or T34-b1.3 α-actin (right panel) were represented vs different cDNA amounts. The corresponding equations and the correlation coefficients are indicated at the upper part of each panel. These calibration curves were used to calculate the amount of cDNA from a given value of Ct, to study the tomato root colonization ability of the different Trichoderma strains studied in the present work.
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