Methanotrophs, methanogens and microbial community structure in livestock slurry surface crusts
Version of Record online: 14 JUL 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 4, pages 1066–1078, October 2014
How to Cite
Duan, Y.-F., Al-Soud, W.A., Brejnrod, A., Sørensen, S.J., Elsgaard, L., Petersen, S.O. and Boon, N. (2014), Methanotrophs, methanogens and microbial community structure in livestock slurry surface crusts. Journal of Applied Microbiology, 117: 1066–1078. doi: 10.1111/jam.12584
- Issue online: 15 SEP 2014
- Version of Record online: 14 JUL 2014
- Accepted manuscript online: 24 JUN 2014 05:39AM EST
- Manuscript Accepted: 17 JUN 2014
- Manuscript Revised: 15 JUN 2014
- Manuscript Received: 22 APR 2014
- Danish Agency for Science, Technology and Innovation
- Geconcerteerde Onderzoeksactie (GOA) of Ghent University. Grant Number: BOF09/GOA/005
Table S1 Sequences of PCR primers used in DGGE and pyrosequencing.
Table S2 Test of specificity for primer sets Type IF/IR and Type IIF/IIR by RDP Probe Match. The test was performed with RDP Release 11, Update 2, 7 March 2014 and with the following parameters: strain = both (type and non-Type), source = isolates, size = both (≥1200 and <1200), quality = good and differences allowed = 0. Only taxa with a positive hit were listed.
Figure S1 Pictures of the surface crusts used in this study taken at time of sampling. Full-scale stores for CS-1 and CS-2 are approximately 30 m in diameter, and pilot-scale stores for SS and SN are approximately 2 m in diameter.
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