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Figure S1. Confocal scanning laser microscope image to indicate bacterial cell attachment onto tailings particles. Bacterial cell within the biofilm samples grown at the tailings–water interface was treated with Syto 9.

Figure S2. Fe 2p X-ray absorption spectra of FeCl2·4H2O, Fe Cl3·6H2O standards and Fe2+-rich location in the biofilm sample grown at 1 m depth.

Table S1. Chemical characterization of the water column covering the DTMF tailings. Measurements were carried out at the time (August, 2011) of biofilm coupon removal. The table is divided into two panels for presentation purposes. Total concentrations of the elements are as mg l−1. Specific conductivity is expressed as μS cm−1.

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