Plant-available inorganic nutrient levels are increased in rice-derived distillery effluents inoculated with microbes
Article first published online: 28 AUG 2014
© 2014 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 117, Issue 5, pages 1412–1421, November 2014
How to Cite
Asano, R., Kobayashi, S., Sonobe, K., Shime-Hattori, A., Okazaki, K. and Ohtomo, R. (2014), Plant-available inorganic nutrient levels are increased in rice-derived distillery effluents inoculated with microbes. Journal of Applied Microbiology, 117: 1412–1421. doi: 10.1111/jam.12600
- Issue published online: 14 OCT 2014
- Article first published online: 28 AUG 2014
- Accepted manuscript online: 17 JUL 2014 03:17AM EST
- Manuscript Accepted: 14 JUL 2014
- Manuscript Revised: 30 JUN 2014
- Manuscript Received: 7 FEB 2014
- distillery effluent;
- liquid fertilizer;
The purpose of this study was to assess the effects of microbes on plant-available inorganic nutrients and a phytohormone in rice-derived distillery effluents.
Methods and Results
The effects of 37 microbial strains on the components of distillery effluents were investigated. Inoculation of several Aspergillus and Bacillus strains resulted in accumulation of a large quantity of ammonium nitrogen (NH4-N; 774 ± 490 and 1059 ± 463 mg l−1, respectively) in the effluent. However, a decrease in the liquid phase during Aspergillus incubation suggested the requirement for additional treatment of the solid residue, whereas the growth of Bacillus subtilis was inhibited by the acidic conditions in the raw distillery effluent. Interestingly, Aspergillus caelatus, Aspergillus oryzae and Aspergillus tamarii yielded greater increases in nitrate concentrations (30–39 mg l−1). Colorimetric and gas chromatography–mass spectrometry analyses revealed that Wickerhamomyces strains generated 7–26 mg l−1 of indole-3-acetic acid (IAA) when the effluent pH was adjusted to 7·0.
Inoculation of several Aspergillus and Bacillus strains into distillery effluents resulted in the production of a large quantity of NH4-N.
Significance and Impact of the study
This study provides information that will facilitate the bioconversion of distillery effluent into fast-acting liquid fertilizers.