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Figure S1. Composite image of black fly peritrophic matrix (PM) colonized by Harpella spp. from Cottonwood Creek (reference site) slide ID-84-E1. Scale bar = 100 μm.

Table S1. Retention time, MRM conditions, qualifier and qualifier ions, average percent recovery of matrix spikes, and instrumental limits of detection (LOD) for pesticides analyzed in larval black fly tissue.

Table S2. Pesticide concentrations (ng/l) in surface water from the two reference (Cottonwood Creek and Dry Creek) and two agricultural (Wanstad Road Ditch and Sand Run Gulch) sites collected between April and December 2010.

Table S3. Composite pesticide concentration (μg/g wet weight) in sampled black fly larvae collected from reference sites (Cottonwood Creek, Dry Creek) and agricultural sites (Sand Run Gulch, Wanstad Ditch).

Table S4. Estimated pesticide concentrations (μg/g wet weight) of individual black fly larvae collected from reference sites (Cottonwood Creek, Dry Creek) and agricultural sites (Sand Run Gulch, Wanstad Ditch).

Table S5. Identified black fly larvae from 2010 samples. N refers to the number of black fly larvae identified from that site over the eight-month sampling period (421 total).

Table S6. Mixed model results for thalli/μm2 and spores/μm2. The log of thalli and spores (plus 5E-07) were modeled, with fixed effect of date, site, and date by site interaction. Due to insufficient numbers of black flies, Wanstad Ditch was not included in the model. Separate residuals were modeled for each date by site combination to accommodate unequal variation.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.