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Chloroplast phylogeography of the East Asian Arcto-Tertiary relict Tetracentron sinense (Trochodendraceae)

Authors

  • Yanxia Sun,

    1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China
    2. University of Chinese Academy of Sciences, Beijing, China
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  • Michael J. Moore,

    1. Department of Biology, Oberlin College, Oberlin, Ohio, USA
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  • Liangliang Yue,

    1. The State Key Laboratory of Species Identification, Yunnan Entry–Exit Inspection and Quarantine Bureau, Kunming, Yunnan, China
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  • Tao Feng,

    1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China
    2. University of Chinese Academy of Sciences, Beijing, China
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  • Haijia Chu,

    1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China
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  • Shaotian Chen,

    1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China
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  • Yunheng Ji,

    1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China
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  • Hengchang Wang,

    Corresponding author
    1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China
    • Correspondence: Hengchang Wang and Jianqiang Li, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.

      E-mails: hcwang@wbgcas.cn; lijq@wbgcas.cn

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  • Jianqiang Li

    Corresponding author
    1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China
    • Correspondence: Hengchang Wang and Jianqiang Li, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.

      E-mails: hcwang@wbgcas.cn; lijq@wbgcas.cn

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Abstract

Aim

A phylogeographical study of the widespread but phylogenetically isolated East Asian endemic tree species Tetracentron sinense (Trochodendraceae) was performed to evaluate whether and how Pleistocene and pre-Pleistocene climate changes helped to influence current phylogeographical patterns, and to describe the current patterns of genetic diversity and their implications for conservation.

Location

Southwestern and central subtropical China.

Methods

Sequences of four chloroplast spacer regions were obtained from 157 individuals of T. sinense. A haplotype network was constructed using tcs. Genetic diversity and differentiation, spatial analysis of molecular variance (SAMOVA) and analysis of molecular variance (AMOVA) were used to test for genetic structure. beast was used to estimate the divergence times between haplotypes. Historical demographic expansion was tested using pairwise mismatch distribution analysis.

Results

Of the 21 recovered haplotypes, three were widely distributed, but most were restricted to particular regions. Populations with high haplotype diversity were located in western Hubei, southern Sichuan and southern Chongqing. The two earliest-diverging haplotypes were found in southwestern China. The haplotype distribution of T. sinense demonstrated significant phylogeographical structure (NST > GST;< 0.05). The best partitioning of genetic diversity by SAMOVA (= 5) produced groups that matched the main tcs-derived clades. Two independent range expansions within SAMOVA-derived groups 2 and 3 were dated to approximately 399 and 311 ka, respectively. The time to the most recent common ancestor of all haplotypes was 9.6 (95% highest posterior density: 27.0–2.2) Ma, but most of the haplotype diversity appeared during the Quaternary.

Main conclusions

The extant distribution of T. sinense is likely to have been shaped by both pre-Quaternary and Pleistocene climate changes. Southwestern China may have served as an important refugium for T. sinense throughout the Neogene, while the species also occupied multiple refugia during the late Pleistocene glacial periods. Populations of T. sinense were resolved into five allopatric groups, between which there is apparently no seed movement.

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