jbi12349-sup-0001-AppendixS1-S3.docWord document654K

Appendix S1 Additional materials. (a) List of the species of the Trechus fulvus group, including distribution and previous hypotheses of relationships. According to the results of this study the species T. arribasi, T. aurouxi and T. gloriensis are excluded from the group. (b) Additional data on the studied specimens: locality data, collector and sequence accession numbers.

Appendix S2 Additional methods: list of primers used for sequencing and standard PCR conditions.

Appendix S3 Additional phylogenetic results. (a) Bayes factor comparison of beast phylogenetic analyses conducted under alternative clock models. (b) Ultrametric time-calibrated tree obtained with beast for the combined dataset with detailed node support values. (c) Ancestral area reconstruction for alternative topologies of node D in Fig. 2.

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