This study was supported in part by National Institutes of Health grants P01 HL78931, R01 HL78932, R01 71140, and R21 HL106554; and by the Laubisch and Kawata Endowments of the University of California Los Angeles (Dr. J. Weiss), a Medtronic-Zipes Endowment of Indiana University (Dr. P. Chen) and the Indiana University Health-Indiana University School of Medicine Strategic Research Initiative.
Apamin-Sensitive Calcium-Activated Potassium Currents in Rabbit Ventricles with Chronic Myocardial Infarction
Article first published online: 29 MAY 2013
© 2013 Wiley Periodicals, Inc.
Journal of Cardiovascular Electrophysiology
Volume 24, Issue 10, pages 1144–1153, October 2013
How to Cite
LEE, Y. S., CHANG, P.-C., HSUEH, C.-H., MARUYAMA, M., PARK, H. W., RHEE, K.-S., HSIEH, Y.-C., SHEN, C., WEISS, J. N., CHEN, Z., LIN, S.-F. and CHEN, P.-S. (2013), Apamin-Sensitive Calcium-Activated Potassium Currents in Rabbit Ventricles with Chronic Myocardial Infarction. Journal of Cardiovascular Electrophysiology, 24: 1144–1153. doi: 10.1111/jce.12176
Medtronic Inc., St. Jude Inc., Cryocath Inc., and Cyberonics Inc. donated research equipment to Dr. Chen's laboratory. Dr. Chen is a consultant to Cyberonics Inc. Other authors: No disclosures.
- Issue published online: 8 OCT 2013
- Article first published online: 29 MAY 2013
- Accepted manuscript online: 7 MAY 2013 06:36AM EST
- Manuscript Accepted: 22 APR 2013
- Manuscript Revised: 13 APR 2013
- Manuscript Received: 6 JAN 2013
- National Institutes of Health. Grant Numbers: P01 HL78931, R01 HL78932, R01 71140, R21 HL106554
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
Figure S1. Effects of apamin on delta APD80 after the ventricles were paced at 200-millisecond PCL of 50–400 beats. In A through C, the first and third rows show action potential at 300-millisecond PCL. The second and forth rows show action potentials during rapid pacing followed by a postpacing beat with S1–S2 coupling interval of 300 milliseconds. The ΔAPD80 was the difference between S2 APD80 and S1 APD80. The same pacing protocol was performed before and after apamin. D through F show effects of apamin on ΔAPD80 increases when the number of paced beats increases. Asterisks indicate P < 0.05 between control and apamin at each PCL.
Figure S2. Western blotting analyses of SK2 proteins. Panel A shows the Western blotting results. Panel B shows the SK2/GAPDH ratio of all 5 hearts studied. There were no differences of the protein ratio in PZ and RZ versus control.
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