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Keywords:

  • β-catenin;
  • Pharmacological inhibitors;
  • Phytochemicals;
  • Cancer

Abstract

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

Beta-catenin (β-catenin) is a multifunction protein with a central role in physiological homeostasis. Its abnormal expression leads to various diseases including cancer. In normal physiology, β-catenin either maintains integrity of epithelial tissues or controls transcription of various genes on extracellular instigations. In epithelial tissues, β-catenin functions as a component of the cadherin protein complex and regulates epithelial cell growth and intracellular adhesion. In Wnt signalling, β-catenin is a major transcriptional modulator and plays a crucial role in embryogenesis, stem cell renewal and organ regeneration. Aberrant expression of β-catenin can induce malignant pathways in normal cells and its abnormal activity is also exploited by existing malignant programmes. It acts as an oncogene and modulates transcription of genes to drive cancer initiation, progression, survival and relapse. Abnormal expression and function of β-catenin in cancer makes it a putative drug target. In the past decade, various attempts have been made to identify and characterize various pharmacological inhibitors of β-catenin. Many of these inhibitors are currently being investigated for their anticancer activities in a variety of cancers. The first half of this review will focus on the role of β-catenin in cancer initiation, maintenance, progression and relapse whereas the second half will briefly summarize the recent progress in development of agents for the pharmacological modulation of β-catenin activity in cancer therapeutics.


Introduction

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

Beta-catenin (β-catenin) is the mammalian homologue of the drosophila armadillo gene [1]. It acts both as a transcriptional co-regulator and an adaptor protein for intracellular adhesion [1-3]. β-catenin is essential for the establishment and maintenance of epithelial layers and provides a mechanical linkage between intracellular junctions and cytoskeletal proteins [4, 5]. Wnt signalling is the chief regulator of β-catenin [6, 7]. Binding of the Wnt ligand to frizzled receptors hyper-phosphorylates and thus activates the dishevelled protein (dsh) [8]. Hyper-phosphorylation of dsh results in the displacement of GSK-3β from the β-catenin degradation complex and prevents GSK-3β-mediated phosphorylation of β-catenin [8]. This complex comprises adenomatous polyposis coli (APC), axin and GSK-3β [8]. In the absence of Wnt signal, GSK-3β and casein kinase 1 (CK1) phosphorylate β-catenin [8, 9]. Phosphorylation of β-catenin leads to its ubiquitination and proteasomal degradation through the F-box/WD repeat-containing protein 1A (FBXW1A)/S-phase kinase-associated protein (SKP) complex [8]. When not degraded via the proteolytic pathway, β-catenin accumulates in the perinuclear region and forms a cytoplasmic pool of free signalling molecules [8, 9]. Here, the stable β-catenin interacts with the lymphoid enhancer factor/T cell factor (LEF/TCF) and is translocated into the nucleus as a complex of β-catenin/LEF/TCF to stimulate target gene transcription by displacement of groucho-HDAC co-repressors [8, 10].

Various extracellular signals regulate the localization of β-catenin either on the membrane or in the cytoplasm [11]. Activation of receptor tyrosine kinases (RTKs) or cytoplasmic tyrosine kinases (Fer, Fyn, Yes and Src), phosphorylate β-catenin at specific tyrosine residues Y654 and Y142 [12]. Y654 phosphorylation leads to the inhibition of the catenin/E-cadherin interaction, leading to the dissociation of the complex and subsequent degradation of E-cadherin and β-catenin [13]. Dissociation of the E-cadherin–β-catenin complex leads to the loss of epithelial apico-basal polarity [14]. At the same time presence of other signals decide cellular response to this change [11]. Extracellular signals mediated through growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) activate PI3K-AKT-MAPK or PKC pathways [15-19]. Activation of these pathways promotes nuclear accumulation of β-catenin by inhibition of GSK3β and supports epithelial to mesenchymal transition (EMT). [15-19]. These pathways also play a critical role in transforming epithelial tumours into an invasive forms and help in the progression of various fibrotic diseases [19]. β-catenin accumulation within the nucleus or cytoplasm has been found in more than half of all cancer cases and is related to increased tumourigenicity [20-25]. Cytoplasmic β-catenin is a hallmark of colon cancer [1]. It can induce tumourigenic traits in normal cells, and further supports cancer cell proliferation and survival [19, 24]. High-level cytoplasmic expression, and nuclear localization of beta-catenin, is characteristic of stem-like cell populations in cancers that are resistant to chemotherapeutics and capable of initiating new tumours [29, 30]. β-catenin also helps in creating a suitable niche for cancer progression by modulating cancer microenvironment [18, 26-30].Various studies have shown that inhibition of β-catenin activity leads to suppression of several cancer hallmarks and is thus perceived as a putative drug target [31].

Role of β-catenin in cancer

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

Accumulating evidence indicates that β-catenin has a central role in the malignant transformation of normal cells [32-36]. Herencia et al. while studying hepatocyte differentiation in mesenchymal stem cells have found that the activation of Wnt signalling and β-catenin nuclear localization results in a tumoural phenotype [32]. They reported an increase in the expression of liver cancer-related proteins in cells with high β-catenin nuclear localization [32]. Heiser et al. observed rapid formation of lipogenic liver tumours in mice on AKT and β-catenin co-activation [33]. In pancreatic cells and lung epithelial cells, activation of β-catenin has also been reported to be sufficient for induction of oncogenic transformation [34, 35]. A recent study demonstrated that Wnt/β-catenin pathway activation in cerebellar progenitor cell prevents terminal differentiation of these cells and maintain them in a stem cell like state [36]. This study further suggested that medulloblastoma can also originate from cells other than granule progenitors. Wnt/β-catenin pathway is also a major regulator of cancer initiating cell (CIC) genesis [36]. Oncogenic mutants of β-catenin have also been reported and the prevention of their degradation results in intracellular accumulation. These mutants can induce tumour formation in transgenic animals [37, 38]. The importance of β-catenin in abnormal cell proliferation attained prominence after the discovery of β-catenin oncogenic mutations in APC wild-type colon cancers [3, 39]. Mutant β-catenin protein is not degraded by APC, thus leading to its accumulation in the cytoplasm resulting in uncontrolled cellular proliferation [39]. The frequency of oncogenic mutations in β-catenin is low but has been reported in a variety of human cancers [40]. Several studies have shown (reviewed elsewhere) that β-catenin is a key modulator of cancer cell proliferation and survival [4, 41]. Initial key studies carried out by Tetsu et al. and Shtutman et al. in colon cancers revealed that β-catenin activates transcription from the cyclin D1 (CCND1) promoter, and consensus TCF/LEF-binding sites are necessary for this activity [42, 43]. β-catenin/TCF/LEF transcription activity also regulates expression of c-Myc, TP63 isoform ΔN (ΔNp63), microphthalmia-associated transcription factor (MITF), limb bud and heart development homolog (LBH), survivin and c-Jun in various cancer models [44-49]. c-MYC and c-JUN act as oncogenes in their active state, while ΔNp63, CCND1, MITF, LBH perform various functions to support cell growth and survival [44-48]. A list of β-catenin target genes in various cancers is briefly summarized in Table 1. β-catenin has also been found to support tumour growth by promoting angiogenesis in cancers [49]. It regulates expression of vascular endothelial growth factor (VEGF) [49]. Collectively, these studies indicate that β-catenin has an important role in maintaining malignancies by supporting cell proliferation and survival.

Table 1. Major β-catenin target genes in cancer
S.NO.Gene NameFunction
1MYCBP [113], JAG1 [114], MSL1 [11], PPARdelta [110],Cell transformation
2CCND1 [42, 43], c-myc [44], ΔNp63 [45], MITF [46], LBH [47], survivin [48] and c-Jun [49], fra-1(Fosl1) [49], FGF18 [107], Hath1 [108], MET [109], FGF9 [112]Cell growth, Proliferation and survival
3MMP2 [51], MMP9 [51], MMP-7 [52], MMP26 [54], VEGF [48], TIAM1[112], TWIST1 [115], SNAI2 [57], ZEB1[116], S100A4 [58], uPAR [49]Migration, Invasion, EMT
4CD44 [104], VEGF [48], BMP4 [106], Ephb [105], GREM1 [110], EDN1[103]Progression, Angiogenesis and Niche establishment
5CD44 [104], HTERT [117], NANOG [117], OCT4 [118]Cancer stem cells

Metastasis is an important cancer hallmark and it is often supported by abnormal β-catenin expression or localization [50]. β-catenin supports the metastatic programme by increasing the migratory and invasive capabilities of cancer cells [18, 42]. It regulates expression of various invasion-related genes like matrix metalloproteinases (MMP2, MMP7, MMP9, MMP26) [51-54]. β-catenin also regulates EMT, which can endow cells with higher invasive, metastatic and survival potential [26]. EMT-like state in cancers is promoted by activation of Snail1 (Snail), Snail2 (Slug), ZEB1, CBF-A/KAP-1 complex, Twist, LEF-1, Ets-1, FOXC2 and Goosecoid transcription factors (TFs) [26]. These TFs work downstream of various growth factor (EGF, TGF-β and IGF1) signalling pathways initiated by changes in cancer microenvironment [55]. Snail and slug transcription factors help in the formation of β-catenin/LEF-1 transcription complex and promote expression of transforming growth factor 3-beta (TGFβ3) to induce EMT [56]. β-catenin/LEF1 also regulates expression of Snail, LEF1 and other EMT markers at the transcriptional level [57]. β-catenin regulates expression of metastasis-associated gene S100A4 and Tenascin C (TNC) [58, 59]. TNC is an extracellular matrix (ECM) protein [60]. It supports the growth and proliferation of metastasis-initiating cancer cells and acts as an important ECM component of the metastatic niche [60]. Nuclear localization of β-catenin has been postulated as a potential marker for local lymph node or distant metastasis in variety of cancers including oesophageal, breast, colorectal, prostate, lung and cervical cancer [61-65]. Apart from the increased expression or nuclear localization a decrease in the β-catenin expression has been observed in melanoma, prostate, thyroid and gastric cancers [66-69]. Decreased β-catenin level in these cancers was associated with their increased metastatic potential [66-69]. This probably indicates that breakdown of normal β-catenin functions can also govern cancer progression and requires further investigation. Cancer cells are associated with various normal stromal cells called cancer-associated cells [70]. Fibroblasts, macrophages, regulatory T cells, mesenchymal stem cells (MSCs) and endothelial cells are the common members of the cancer stroma [70]. These cells in the cancer microenvironment support cancer growth and progression. In oesophageal cancers, tumour-associated fibroblasts are responsible for higher expression and nuclear localization of β-catenin in adjacent cancer cells [71]. A recent study indicated that nuclear overexpression of β-catenin in tumour-associated fibroblasts is a good prognostic indicator in breast cancers [72]. The study also reported that the ectopic expression of β-catenin in fibroblast increases proliferation and invasion of co-cultured cancer cells [72]. Fibroblasts in co-culture have also been shown to increase expression of β-catenin in breast cancer cells [73]. It also increases proliferation of CD44+/CD24low/- (CSC) subpopulation to a fivefold higher level than that of the normal breast cancer cells [73]. These studies underscore the importance of β-catenin in regulating tumour microenvironment. However, the low β-catenin expression associated with metastasis needs further investigation. Collectively, β-catenin activity is modulated by extracellular changes and in response it modulates cancer microenvironment to promote tumour growth, invasion and metastasis [18, 55].

Abnormal activity of β-catenin is further associated with cancer drug resistance and cancer stem cell state [29, 30]. It thus associated with poor patient outcome and disease relapse [29, 30, 74-77]. β-catenin is essential for the self-renewal of normal as well as cancer stem cells. Zhao et al. explored the role of β-catenin in haematopoietic malignancies [74]. They found that β-catenin plays an essential role in AML and CML development and also helps in cancer stem cell renewal [74]. Various other studies have postulated that angiogenesis, presence of highly resistant cancer stem cells (CSCs), EMT, deregulation of cell cycle and apoptosis are central wheels in mechanisms of cancer aggressiveness and chemoresistance [78, 79]. Current knowledgebase suggests that Wnt/β-catenin signalling has a role in all these five aspects associated with the process of carcinogenesis. It plays an essential role in cancer initiation, maintenance, progression, survival and relapse [18, 26, 32, 57, 74-76]. Owing to its place at the heart of malignant programmes, β-catenin is increasingly perceived as a putative drug target (Fig. 1).

image

Figure 1. Role of β-catenin in Tumourigenesis. Beta-catenin supports: (A) transformation of normal cells to cancerous one. (B) Cancer cell proliferation, renewal, differentiation, niche establishment, angiogenesis and EMT. (C) Invasion and Intravasion. (D) Extravasion. (E) tissue invasion and organ homing to establish micrometastasis. CSC, Cancer stem cell; EMT, Epithelial to mesenchymal transition; ECM, Extracellular matrix.

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Beta-catenin inhibitors

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

Inhibition of β-catenin using small molecule inhibitors or siRNA abrogates tumour growth [80, 81]. In the year 2002, Kim et al. for the first time demonstrated that specific inhibition of the oncogenic form of β-catenin is sufficient to reverse the transformed properties of human cancer cells [82]. In their study, they found that β-catenin is a necessary oncogene and the pharmacological inhibition of oncogenic β-catenin is likely to be an effective strategy for reversing the malignant properties of advanced human tumours [82]. To date, many β-catenin signalling pathway inhibitors are under investigation with the potential aim of disrupting β-catenin activity and its interaction with the transcription factors. Lepourcelet et al. made initial attempts to screen and identify compounds capable of disrupting TCF/β-catenin complexes [83]. They screened chemical libraries of small molecules using a high-throughput assay system and found two potent inhibitors (PKF115-584 and PKF222-815) capable of disrupting TCF/β-catenin complexes and antagonize cellular effects of β-catenin-dependent activities [83]. They also identified other β-catenin inhibitors (PKF118-310, CGP049090 and PKF118-744) capable of inhibiting β-catenin activity [83]. Furthermore, Wnt/β-catenin signalling inhibitor PKF118–310 effectively inhibited proliferation of prostate cancer cells (IC50 ≤ 1 μM) [84]. Minke et al. and Gandhirajan et al. investigated the effects of CGP049090 (IC50 ≤ 1 μM) and PFK115-584 (IC50 ≤ 1 μM) in acute myeloid leukaemia (AML) and chronic lymphocytic leukaemia (CLL) cells respectively [85, 86].They found that both compounds led to a substantial decrease in the expression of β-catenin/LEF1 target genes (e.g. c-myc, cyclin D1 and survivin). Down-regulation of these survival-related genes resulted in the induction of cell death in AML cell lines and cells derived from AML patients [86]. These inhibitors also induced cell death in CLL cell lines and patient samples [87]. PKF118-310 was also found to be effective against human osteosarcoma cells. Here, inhibition of β-catenin resulted in suppression of MMP9 enzymatic activity and thus reduced cancer cell invasion and migration [87]. Apart from its anti-invasive effects, PKF118-310 also induced cell death and G2/M phase arrest in osteosarcoma cells by decreasing expression of cyclin D1, c-Myc and survivin [87]. Hallett et al. found that PKF118-310 (IC50 ≤ 1 μM) was effective against breast cancer initiating cells (BTIC) where it inhibited tumour growth and proliferation [88]. Administration of PKF118-310 to tumour-bearing mice halted tumour growth in vivo and viable tumour cells harvested from PKF118-310 treated mice were unable to induce the growth of secondary tumours after transplantation [88]. Emami et al. identified a novel inhibitor (ICG-001; IC50 ≤ 3 μM) of beta-catenin/CREB-binding protein transcription activity. ICG-001 induced apoptosis in transformed cells selectively and also reduced in vitro and in vivo growth of colon carcinoma cells [89]. In another attempt to identify novel inhibitors of the Wnt/β-catenin pathway, Ewan et al. screened a chemical library against a transcription factor reporter cell line in which the activity of the pathway was induced at the level of dishevelled (dsh) protein [90]. They identified a potent inhibitor CCT036477 (IC50 ≤ 5 μM), capable of inhibiting TCF/β-catenin-mediated transcription and inducing cancer cell death [90]. Chen et al. identified nine potent β-catenin inhibitors (IC50 ≤ 2.5 μM) [91]. They screened over 200 thousand compounds in vitro to identify less toxic and highly selective inhibitors against the Wnt/β-catenin signalling pathway [91]. Based on the results using cellular systems, five compounds were found to inhibit Wnt response (IWR) and four compounds were found to inhibit Wnt production (IWP) [91]. Huang el al. identified a novel inhibitor (XAV939) which antagonized Wnt/β-catenin pathway by inhibiting tankyrase [92]. Tankyrase is an axin inhibitor, thus XAV939 increases axin levels in cells [92]. Axin stabilization further leads to β-catenin degradation and Wnt/β-catenin pathway inhibition [92]. Song et al. employed a high-throughput screen to identify inhibitors of Wnt/β-catenin signalling [93]. They found a special class of compounds (acyl hydrazones; IC50 ≤ 2 μM) with iron chelating activity [93]. They demonstrated that their inhibitory effect on the Wnt/β-catenin signalling pathway is linked to iron chelation [93]. These results further supported the initial finding of Brookes et al. that iron can induce Wnt/β-catenin signalling [93, 94]. Recently, Coombs et al. used a cell-based assay system as well as transgenic MMTV-Wnt1 and MMTV-PyMT mice models to screen Wnt/β-catenin inhibitors [95]. They found a compound N-((8-hydroxy-7-quinolinyl) (4-methylphenyl)methyl)benzamide (HQBA) with IC50 ranging between <1 nM and 50 μM in various cellular models [95]. In mice models, it effectively reduced tumour mass [95]. HQBA was found to be safe at higher doses (60–90 mg/kg) and interestingly its anticancer effects were also caused by iron chelation [95]. In various other attempts to identify β-catenin inhibitors, many potent compounds capable of inhibiting β-catenin activity as well as its molecular interactions were identified. Some of these inhibitors are listed in Table 2.

Table 2. Small molecular inhibitors of β-catenin signaling
S.no.InhibitorTargetReference
1PKF118-310, CGP049090, PKF115-584, PKF222-815 and PKF118-744β-catenin–TCF interaction [83]
2ICG001β-catenin–CBP interaction [89]
3CCT036477β-catenin–TCF interaction [90]
4XAV939Tankyrase [92]
5Acyl hydrazones, HQBAIron chelators [93, 95]
6Molecules with 2,3,6-trisubstituted pyrido[2,3,-b] pyrazine core skeletonsβ-catenin [119]
7Carnosic acidβ-catenin/BCL9 [120]
8CCT031374β-catenin [121]
9iCRT-3,5,14, NC043β-catenin–TCF interaction [122, 123]
10Ibuprofin, aspirinCox2 Inhibitors [124]

Furthermore, inhibition of β-catenin can also be employed against cancer stem cells and chemo-resistant cancer cells. The Rosen laboratory evaluated radiation resistance in CSCs isolated from p53-null mouse mammary tumours [96]. Using the inhibitor perifosine, they were able to block AKT and β-catenin activation and sensitize the cells to radiation [81]. Another study has shown that β-catenin is a target of selenium and its inhibition is associated with increased chemosensitivity to cytotoxic drugs in various human cancers [96]. However, there are only limited reports detailing the toxicological, pharmacokinetic and pharmacodynamic data for these inhibitors. Collectively, the studies carried out using small molecule inhibitors of β-catenin targeted to inhibit cancer progression look promising. These small molecule inhibitors reduce cancer growth, induce apoptosis, decrease invasion and migration of cancer cells.

Plant-derived beta-catenin modulators

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

Various plant-derived compounds with anticancer activities are also known to inhibit or modulate the Wnt/β-catenin signalling pathway. Tetrandrine (TET), a bis-benzylisoquinoline alkaloid purified from the root of Stephania tetrandra exhibited significant anticancer activity by inhibiting β-catenin/Tcf transcriptional activity (IC50 range, 1.25–5.7 μM) [97]. Curcumin, a plant-derived natural phenol from the popular Indian spice turmeric shows excellent tumour inhibition property without significant toxicity [98]. Curcumin and its derivative CHC007100 inhibit β-catenin/Tcf signalling by 58–63% and 70–78%, at 20 and 100 μM doses respectively [98]. Another plant-derived flavonoid quercetin also leads to the decrease in beta-catenin/Tcf transcriptional activity [99]. Quercetin acts at a very high dose (IC50, 100 μM) and further investigation is required for data related to its safety and efficacy. Plant flavanoid silymarin, from Silybum marianum, inhibits melanoma cell migration (IC50 ≤ 20 μM) by inhibiting β-catenin nuclear localization [100]. Carnosol, from the herb rosemary, prevents APC-associated intestinal tumourigenesis in a mouse model of colonic tumourigenesis. Its dietary administration (0.1%) reduced tumour growth by 46 per cent without any toxicity. It suppressed tumour growth via its ability to enhance E-cadherin-mediated adhesion and inhibition of β-catenin tyrosine phosphorylation [101]. Cardamonin a natural compound derived from Aplinia katsumadai inhibits 65–70 per cent of β-catenin activity at a dose ≤10 μM, without compromising cell viability [102]. These studies indicate that various plant-derived chemicals (phytochemicals) and their various analogues can also modulate β-catenin functions and thus could be tested against various cancers with abnormal β-catenin activity. These phytochemicals and their derivatives further require thorough investigation for their safety and efficacy.

Conclusions and perspectives

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

The potential of the pharmacological modulation of β-catenin in cancer therapeutics is paramount. This may possibly provide an attractive option of targeting various aspects of the carcinogenic process i.e. initiation, progression and chemoresistance in conjunction with the traditional chemotherapy. However, the long-term effects of the pharmacological manipulation of β-catenin remain still unclear. The overall regulation of β-catenin involves multiple signalling pathways and therefore pharmacological modulation could be counterbalanced through the activation of compensatory signalling pathways. The possibility of adverse side effects of β-catenin inhibition cannot be ruled out at this juncture and more detailed studies will be required to address this key issue. To date, use of various small molecule inhibitors of β-catenin targeting cancer have provided some encouraging results. Further efforts can be directed towards evaluating the efficacy of the existing inhibitors in variety of cancer types, stages and especially against cancer initiating cells/cancer stem cells and chemo-resistant cancers. As it is evident that microenvironmental regulation of the β-catenin activity plays a central role in the malignant transformation and induction of metastasis; these inhibitors can also be used in combination with inhibitors of cancer survival pathways and modulators of tumour microenvironment. Some of the phytochemicals that seem to modulate β-catenin activity can also be used as lead compounds for developing β-catenin-targeted therapeutics. Targeting Wnt–β-catenin activity could open new avenues for novel and tailor-made cancer therapeutic approaches.

Acknowledgements

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References

We wish to apologize to all colleagues whose work, because of lack of space, could not be cited. We thank all the members of the DP Mishra and S Musthapa Meeran laboratories for helpful discussions. This work was supported by the grants from the Defense Research and Development Organization (GAP-0058) to DP Mishra. Ravi Thakur acknowledges the support by the junior research fellowship from the Council of Scientific and Industrial Research, New Delhi. The CSIR-CDRI Communication Number of this manuscript is 8407.

References

  1. Top of page
  2. Abstract
  3. Introduction
  4. Role of β-catenin in cancer
  5. Beta-catenin inhibitors
  6. Plant-derived beta-catenin modulators
  7. Conclusions and perspectives
  8. Acknowledgements
  9. Conflict of interest
  10. References