jcmm12038-sup-0001-FigS1.tifimage/tif1559KFigure S1 Immunohistochemistry of M2 receptors in fresh glioma biopsy. The arrows indicated the positive cells (1000X).
jcmm12038-sup-0002-FigS2.tifimage/tif2718KFigure S2 Immunocytochemistry analysis of M2 receptor in U251 cells after siRNA transfection. (A) Control (in absence of siRNA). The red arrows indicate the positive cells (150x). (B) Hoechst staining of U251 siRNA transfected cells; the blue arrows indicate the nuclei of cells that are completely negative for M2 staining in Fig. C (150x). (C) M2 staining in U251 siRNA transfected cells (150x). The immunostaining appears decreased respect to the control (A); some spots of fluorescence appear on the plasma membrane of the cells, as evident in the frame showing higher magnification of one cell (300x).

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