Telocytes: new insight into the pathogenesis of gallstone disease
Article first published online: 4 APR 2013
© 2013 The Authors Journal of Cellular and Molecular Medicine Published by Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
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Journal of Cellular and Molecular Medicine
Volume 17, Issue 6, pages 734–742, June 2013
How to Cite
Matyja, A., Gil, K., Pasternak, A., Sztefko, K., Gajda, M., Tomaszewski, K. A., Matyja, M., Walocha, J. A., Kulig, J. and Thor, P. (2013), Telocytes: new insight into the pathogenesis of gallstone disease. Journal of Cellular and Molecular Medicine, 17: 734–742. doi: 10.1111/jcmm.12057
- Issue published online: 20 JUN 2013
- Article first published online: 4 APR 2013
- Manuscript Accepted: 1 MAR 2013
- Manuscript Received: 15 DEC 2012
- interstitial Cajal-like cells;
- cholesterol saturation index;
- bile lithogenicity
The major mechanisms of gallstone formation include biliary cholesterol hypersecretion, supersaturation and crystallization, mucus hypersecretion, gel formation and bile stasis. Gallbladder hypomotility seems to be a key event that triggers the precipitation of cholesterol microcrystals from supersaturated lithogenic bile. Telocytes, a new type of interstitial cells, have been recently identified in many organs, including gallbladder. Considering telocyte functions, it is presumed that these cells might be involved in the signalling processes. The purpose of this study was to correlate the quantity of telocytes in the gallbladder with the lithogenicity of bile. Gallbladder specimens were collected from 24 patients who underwent elective laparoscopic cholecystectomy for symptomatic gallstone disease. The control group consisted of 25 consecutive patients who received elective treatment for pancreatic head tumours. Telocytes were visualized in paraffin sections of gallbladders with double immunofluorescence using primary antibodies against c-Kit (anti-CD117) and anti-mast cell tryptase. Cholesterol, phospholipid and bile acid levels were measured in gallbladder bile. The number of telocytes in the gallbladder wall was significantly lower in the study group than that in the control group (3.03 ± 1.43 versus 6.34 ± 1.66 cell/field of view in the muscularis propria, P < 0.001) and correlated with a significant increase in the cholesterol saturation index. The glycocholic and taurocholic acid levels were significantly elevated in the control subjects compared with the study group. The results suggest that bile composition may play an important role in the reduction in telocytes density in the gallbladder.