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jcmm12068-sup-0001-FigureS1.tifimage/tif115KFigure S1. Quantitative RT-PCR for Notch1 expression in the eight cell lines. Overexpression of Notch1 was detected in all of the eight lung cancer cell lines at the mRNA level. Normal lung was used as a negative control.
jcmm12068-sup-0002-FigureS2.tifimage/tif362KFigure S2. Immunohistochemistry Notch1 staining of three additional NSCLC cell lines. Three different NSCLC cell lines express dramatically different levels of Notch1 protein. H157 cell line expressed minimal level of Notch1 protein, SW900 cell line expressed medium level of Notch1 protein, and H520 NSCLC cell line expressed high level of Notch1 protein. Images were taken with a 40 ×  magnification.
jcmm12068-sup-0003-FigureS3.tifimage/tif52KFigure S3. Effect of Notch1 by CK2α siRNA in A427 cells. CK2α expression was silenced using siRNA knockdown in A427 cell line. The corresponding protein level of CK2α decreased dramatically (upper lane). The protein level of Notch1 was not affected after CK2α knockdown in A427 cells with an activating β–catenin mutation (middle lane). Endogenous GAPDH was used as a loading control (lower lane).
jcmm12068-sup-0004-FigureS4.tifimage/tif4508KFigure S4. The Phosphorylation Prediction Results of Human Notch1 with CK2. Two CK2 phosphorylation sites in Notch1 were predicted using Scansite 2.0 with medium stringency.
jcmm12068-sup-0005-TableS1.docxWord document20KTable S1. Study population and specimens. Expression of Notch1 was detected in 41 NSCLC tumours using immunohistochemistry. Intensity of Notch1 staining was scored from low (−) to high (+++). Information of study population was collected: pathology, stages of tumour, ages of the patients at diagnosis and smoking history of the patients. X, the information is unknown.

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