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jcmm12077-sup-0001-FigS1.tifimage/tif7915KFigure S1 The effect of H2S on NO production in primarily cultured mouse aortic endothelial cells. (A) The effects of CSE siRNA knockdown and NaHS treatment on NO production detected by Griess assay, n = 3, *P < 0.05 versus control, #P < 0.05 versus NaHS-treated group. (B) The effects of NaHS and L-NAME treatment on NO production detected by diaminofluorescein diacetate–based probes (DAF-FM), scale bars: 200 μm.
jcmm12077-sup-0002-FigS2.tifimage/tif7915KFigure S2 The pro-proliferative effects of H2S and NO on primarily cultured mouse aortic endothelial cells. (A) The effects of CSE siRNA, eNOS siRNA and NaHS treatments on EC proliferation assessed using BrdU proliferation assay, n = 3, *P < 0.05 versus control, #P < 0.05 versus NaHS-treated group (B) The efficiency of CSE siRNA and eNOS siRNA in ECs detected by Western blot.

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