jcmm12079-sup-0001-FigS1.tifimage/tif711KFigure S1 H2O2 causes induction of Il-13 and TGFβ, whereas the Il-6 and Il-8 release remained nearly unchanged. JNK inhibition using SP600125 led to decreased levels of Il-6 and TGFβ, but to increased levels of Il-8 and Il-13. Following inhibition of caspase activity (Z-VAD-FMK), release of IL-13 was abolished, and that of TGFβ was decreased.
jcmm12079-sup-0002-FigS2.tifimage/tif61KFigure S2 (A) H2O2 results in the up-regulation and cleavage of pro-caspase 3. H2O2 induced caspase 3 expression after 24 hrs, whereas its expression was reversed after 48 and 72 hrs. (B) A solvent effect of DMSO on the decreased caspases expression following JNK inhibition could be excluded. Immunoblot analysis following JNK inhibition by the JNK inhibitor SP600125, compared with the combined DMSO and H2O2 control, revealed caspases 3, 8 and 9 as JNK-regulated proteins.
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