These authors contributed equally.
Non-apoptotic function of caspases in a cellular model of hydrogen peroxide-associated colitis
Article first published online: 7 JUN 2013
© 2013 The Authors. Journal of Cellular and Molecular Medicine Published by Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Journal of Cellular and Molecular Medicine
Volume 17, Issue 7, pages 901–913, July 2013
How to Cite
Poehlmann, A., Reissig, K., Just, A., Walluscheck, D., Hartig, R., Schinlauer, A., Lessel, W., Guenther, T., Silver, A., Steinberg, P. and Roessner, A. (2013), Non-apoptotic function of caspases in a cellular model of hydrogen peroxide-associated colitis. Journal of Cellular and Molecular Medicine, 17: 901–913. doi: 10.1111/jcmm.12079
- Issue published online: 24 JUL 2013
- Article first published online: 7 JUN 2013
- Manuscript Accepted: 15 APR 2013
- Manuscript Received: 3 AUG 2012
|jcmm12079-sup-0001-FigS1.tif||image/tif||711K||Figure S1 H2O2 causes induction of Il-13 and TGFβ, whereas the Il-6 and Il-8 release remained nearly unchanged. JNK inhibition using SP600125 led to decreased levels of Il-6 and TGFβ, but to increased levels of Il-8 and Il-13. Following inhibition of caspase activity (Z-VAD-FMK), release of IL-13 was abolished, and that of TGFβ was decreased.|
|jcmm12079-sup-0002-FigS2.tif||image/tif||61K||Figure S2 (A) H2O2 results in the up-regulation and cleavage of pro-caspase 3. H2O2 induced caspase 3 expression after 24 hrs, whereas its expression was reversed after 48 and 72 hrs. (B) A solvent effect of DMSO on the decreased caspases expression following JNK inhibition could be excluded. Immunoblot analysis following JNK inhibition by the JNK inhibitor SP600125, compared with the combined DMSO and H2O2 control, revealed caspases 3, 8 and 9 as JNK-regulated proteins.|
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