jcmm12087-sup-0001-SupplementalData.docxWord document25KData S1 Supplementary data.
jcmm12087-sup-0002-FigS1.tifimage/tif7293KFigure S1 Cases of slug overexpression showed a reduced E-cadherin expression pattern and an increased vimentin expression pattern in HCC patients.
jcmm12087-sup-0003-FigS2.tifimage/tif2721KFigure S2 Huh7 cells transfected with slug showed CD133+ and CD90+ phenotype, EMT phenotype, VEGF and VE-cadherin expression and VM formation.
jcmm12087-sup-0004-FigS3.tifimage/tif7394KFigure S3 (A) The decreased expression of slug protein in the slug siRNA-treated SMMC-7721 cells (S: SMMC-7721; si S: SMMC-7721 with slug silencing) was evident from the Western blot data. Slug silencing inhibited tube formation (VM) in SMMC-7721 cells in vitro. (B-Q) SMMC-7721 with slug silencing xenografts reversed CSCs and EMT phenotype, inhibited vasculogenic mimicry. Positive slug (B), CD90 expression (D), the loss of E-cadherin expression (F), positive vimentin expression (H), higher VE-cadherin (L) and VEGF (N) expression present in S (SMMC-7721) xenograft. In contrast, the decreased slug (C) and CD90 expression (E), the increased E-cadherin expression (G), and the decreased vimentin (I), VE-cadherin (M) and VEGF (O) present in si S (SMMC-7721 with slug silencing) xenograft. There was VM (black arrow indicated VM channel; green arrow indicated red blood cell; red arrow indicated endomucin-positive blood vessel) present in SMMC-7221 xenograft (J). However, in si S (SMMC-7721 with slug silencing) xenograft, no VM channels were found (K). HLA-DR-positive tumour cells formed VM channel (black arrow indicated VM; red arrow indicated red blood cell) in SMMC-7221 xenograft (P). However, in si S (SMMC-7721 with slug silencing) xenograft, no VM channels were found (Q).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.