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jcmm12097-sup-0001-FigS1.tifimage/tif11237KFigure S1. (A) Different fluorescence signals detected in adenovirus-transduced soft tissues (a) versus calcein-stained new bone formation (b). (B) Immunohistochemical staining analysis indicates that the humerus growth plate from BMP9+EGF-treated limb explants exhibited higher PCNA-positive cells than that of the BMP9-treated alone (63% versus 32%).
jcmm12097-sup-0002-FigS2.tifimage/tif6119KFigure S2. EGF enhances BMP9-induced phosphorylation of Smad1/5/8. Subconfluent iMEFs were infected with GFP, BMP9, EGF and BMP9+EGF adenoviruses. At 24 hrs after infection, cells were fixed and immunostained with a p-Smad1/5/8 antibody (Santa Cruz Biotechnology). Positively stained cells were indicated by arrows. Representative results are shown.
jcmm12097-sup-0003-TablesS1.xlsapplication/msexcel22KTable S1. Oligonucleotides used for cloning, RT-PCR and ChIP.

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