Effects of Cystamine on antioxidant activities and regulatory T cells in lupus-prone mice

Authors

  • Tsai-Ching Hsu,

    1. Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan
    2. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
    3. Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan
    4. Department of Healthcare Administration, Asia University, Taichung, Taiwan
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  • Chun-Ching Chiu,

    1. Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan
    2. Department of Neurology and Department of Medical Intensive Care Unit, Chunghua Christian Hospital, Chunghua, Taiwan
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  • Yi-Wen Wang,

    1. Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan
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  • Bor-Show Tzang

    Corresponding author
    1. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
    2. Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan
    3. Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
    • Correspondence to: Bor-Show TZANG, Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan. No. 110, Sec. 1, Jianguo N. Rd., Taichung 402, Taiwan.

      Tel.: +886-4-24730022 ext. 11680

      Fax: +886-4-23248195

      E-mail: bstzang@csmu.edu.tw

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Errata

This article is corrected by:

  1. Errata: Corrigendum Volume 18, Issue 3, 554, Article first published online: 27 February 2014

Abstract

Attenuated antioxidant activities, irregular cytokines expressions and reduced regulatory T cells, are strongly associated with the pathogenesis of systemic lupus erythematosus (SLE). Despite the well-established beneficial effects of cystamine on lupus-prone mice, the extent to which cystamine contributes to antioxidant activity and the reduction of regulatory T cells has seldom been investigated. Therefore, this study elucidates how cystamine affects anti-oxidant activities in NZB/W F1 mice by performing assays of Glutathione (GSH), 1,1-diphenyl-2- picryl-hydrazyl (DPPH) and malondialdehyde thiobarbituric acid (MDA). In addition, investigations of the effects of cystamine on CD4+/CD25+ regulatory T cells and interleukin-6 (IL6)/STAT-3 signalling were performed with flow cytometry and immunoblots. Experimental results reveal more significantly reduced MDA and increased GSH and DPPH in NZB/W F1 mice receiving cystamine than in those mice receiving PBS. Meanwhile, CD4+/CD25+ regulatory T cells more significantly increase in NZB/W F1 mice receiving cystamine than in those mice receiving PBS, accompanied by significantly reduced IL-6/phosphorylated STAT-3 expression. The above findings suggest the beneficial effects of cystamine in terms of increasing antioxidant activities and CD4+/CD25+ regulatory T cells in lupus-prone mice by suppressing IL-6/STAT3 signalling.

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