Cerebral ischaemia and matrix metalloproteinase-9 modulate the angiogenic function of early and late outgrowth endothelial progenitor cells
Version of Record online: 14 AUG 2013
© 2013 The Authors. Journal of Cellular and Molecular Medicine Published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Journal of Cellular and Molecular Medicine
Volume 17, Issue 12, pages 1543–1553, December 2013
How to Cite
Morancho, A., Hernández-Guillamon, M., Boada, C., Barceló, V., Giralt, D., Ortega, L., Montaner, J. and Rosell, A. (2013), Cerebral ischaemia and matrix metalloproteinase-9 modulate the angiogenic function of early and late outgrowth endothelial progenitor cells. Journal of Cellular and Molecular Medicine, 17: 1543–1553. doi: 10.1111/jcmm.12116
- Issue online: 25 DEC 2013
- Version of Record online: 14 AUG 2013
- Manuscript Accepted: 17 JUL 2013
- Manuscript Received: 20 MAR 2013
- Instituto de Salud Carlos III. Grant Number: PI10/00694
- European Regional Development Fund
|jcmm12116-sup-0001-FigS1-S7.docx||Word document||3489K||Figure S1. Method for culturing EPCs: Mononuclear cells (MNC) were isolated from spleen (mouse) or blood (human) by Ficoll gradient density and seeded in fibronectin (FN)-coated plates with EGM-2 medium. Figure S2. Genotype characterization of wild-type (WT) and knockout (MMP-9/KO) mice. Figure S3. Immunocytochemistry of cobblestone-type mouse OECs. Figure S4. Representative microscopy images of time-lapse imaging assay of mouse OECs on Matrigel™ matrix at different time-points. Figure S5. Gelatin zymography of conditioned media (CM) detected gelatinolytic activity of pro-MMP-9 in media from WT sham and ischaemic EPC. Figure S6. Cell viability determined by MTT assay after MMP inhibitors or vehicle treatment. Figure S7. Brain vasculature in WT and MMP-9/KO mice after cerebral ischaemia.|
|jcmm12116-sup-0002-VideoS1.avi||video/avi||9495K||Video S1. Twenty-four hours of time-lapse imaging MatrigelTM assay of mouse WT control OECs (100×).|
|jcmm12116-sup-0003-VideoS2.avi||video/avi||9496K||Video S2. Twenty-four hours of time-lapse imaging MatrigelTM assay of mouse WT ischaemic OECs (100×).|
|jcmm12116-sup-0004-VideoS3.avi||video/avi||9496K||Video S3. Twenty-four hours of time-lapse imaging MatrigelTM assay of mouse MMP-9/KO control OECs (100×).|
|jcmm12116-sup-0005-VideoS4.avi||video/avi||9496K||Video S4. Twenty-four hours of time-lapse imaging MatrigelTM assay of mouse MMP-9/KO ischaemic OECs (100×).|
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