These authors contributed equally to the present paper.
Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model
Article first published online: 12 SEP 2013
© 2013 The Authors. Journal of Cellular and Molecular Medicine Published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Journal of Cellular and Molecular Medicine
Volume 17, Issue 11, pages 1434–1443, November 2013
How to Cite
Liu, J., Hjorth, E., Zhu, M., Calzarossa, C., Samuelsson, E.-B., Schultzberg, M. and Åkesson, E. (2013), Interplay between human microglia and neural stem/progenitor cells in an allogeneic co-culture model. Journal of Cellular and Molecular Medicine, 17: 1434–1443. doi: 10.1111/jcmm.12123
- Issue published online: 23 DEC 2013
- Article first published online: 12 SEP 2013
- Manuscript Accepted: 27 JUL 2013
- Manuscript Received: 28 MAR 2013
- ALF Research Grant
- Stockholm County Council
- Chinese Scholarship Council
- Magnus Bergvall Foundation
- Svenska Läkaresällskapet
- Karolinska Institutet research funds
- The Stockholms Sjukhem Foundation
- The Swedish Research Council
- Stiftelsen för gamla tjänarinnor
- Bertil Stohnes Stiftelse
- The European Union Seventh Framework Program. Grant Number: 276130
|jcmm12123-sup-0001-FigureS1.jpg||image/jpg||385K||Figure S1. Representative phase contrast microphotographs of mono- and co-cultures at day 7 are shown. Scale bar = 200 μm.|
|jcmm12123-sup-0002-FigureS2.jpg||image/jpg||1368K||Figure S2. Flow cytometry analysis of human NPC death and phenotype in the cultures with higher density of NPCs or co-cultures of NPCs and human foreskin fibroblast. The percentage of cells immunoreactive for (A) 7-AAD, (B) nestin, PSA-NCAM, A2B5, β-tubulin III and GFAP out of the total NPC populations (n ≥ 4/group) was evaluated by anova with Turkey post hoc test. Mean values ± SEM.|
|jcmm12123-sup-0003-FigureS3.jpg||image/jpg||228K||Figure S3. Representative confocal microphotograph of fluorescent latex beads (green) in microglia at day 7 is shown. Nuclei are labelled in blue (Hoechst). Scale bar = 20 μm.|
Table S1 Antibodies used in flow cytometry.
Table S2 Antibodies used in immunocytochemistry.
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