Investigation into the prevalence of a novel dendritic-like cell subset in vivo
Article first published online: 19 NOV 2013
© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Journal of Cellular and Molecular Medicine
Volume 17, Issue 12, pages 1608–1618, December 2013
How to Cite
Griffiths, K. L., Tan, J. K. H. and O'Neill, H. C. (2013), Investigation into the prevalence of a novel dendritic-like cell subset in vivo. Journal of Cellular and Molecular Medicine, 17: 1608–1618. doi: 10.1111/jcmm.12174
- Issue published online: 25 DEC 2013
- Article first published online: 19 NOV 2013
- Manuscript Accepted: 2 OCT 2013
- Manuscript Received: 10 JUL 2013
- National Health and Medical Research Foundation of Australia. Grant Number: 585443
- Dendritic cells;
- bone marrow
A novel dendritic-like cell subset termed L-DC was recently identified in murine spleen based on marker expression of a homogeneous cell population derived from long-term culture of neonatal spleen. The function of L-DC is distinct from other splenic dendritic and myeloid cell subsets because of their high endocytic capacity and their ability to cross-present antigen to CD8+ T cells. This paper shows the subset to be unique to spleen and blood, with a similar, but possibly functionally distinct subset also present in bone marrow. The prevalence of the subset is low; ~6% of all dendritic and myeloid cells in the spleen and ~5% in blood. However, they are a distinct cell type on the basis of marker expression, and endocytic and T-cell stimulatory capacity. Attempts to identify an enriched population of these cells in mutant mouse strains with reported increases in myelopoiesis showed either a lack of L-DC or an altered phenotype reflective of the phenotype of the mouse strain.