Modulation of adipocyte differentiation by omega-3 polyunsaturated fatty acids involves the ubiquitin-proteasome system
Article first published online: 29 JAN 2014
© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Journal of Cellular and Molecular Medicine
Volume 18, Issue 4, pages 590–599, April 2014
How to Cite
Wójcik, C., Lohe, K., Kuang, C., Xiao, Y., Jouni, Z. and Poels, E. (2014), Modulation of adipocyte differentiation by omega-3 polyunsaturated fatty acids involves the ubiquitin-proteasome system. Journal of Cellular and Molecular Medicine, 18: 590–599. doi: 10.1111/jcmm.12194
- Issue published online: 8 APR 2014
- Article first published online: 29 JAN 2014
- Manuscript Accepted: 23 OCT 2013
- Manuscript Received: 13 MAY 2013
- FA: Mead Johnson Nutrition/IU School of Medicine
- EPA ;
- ω-3 polyunsaturated fatty acids;
- fatty acid synthase;
- 3T3-L1 cells
We have evaluated the effects of three different omega-3 polyunsaturated fatty acids (ω-3 PUFAs) – docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and docosapentaenoic acid (DPA) on fat accumulation and expression of adipogenic and inflammatory markers using both 3T3-L1 pre-adipocytes and differentiated 3T3-L1 adipocytes. Our results indicate that ω-3 PUFAs induce the degradation of fatty acid synthase through the ubiquitin-proteasome system, which is likely to have beneficial metabolic effect on adipose cells. Omega-3 PUFAs also increase overall levels of polyubiquitinated proteins, at least in part through decreasing the expression of proteasome subunits. Moreover, adipocytes are resistant to proteasome inhibition, which induces adipophilin while decreasing perilipin expression. On the other hand, ω-3 PUFAs decrease expression of SREBP1 while inducing expression of adipophilin and GLUT4. Moreover, all three ω-3 PUFAs appear to induce tumour necrosis factor-α without affecting NFκB levels. All three ω-3 PUFAs appear to have overall similar effects. Further research is needed to elucidate their mechanism of action.