These authors contributed equally to this work.
Serum and supplement optimization for EU GMP-compliance in cardiospheres cell culture
Article first published online: 20 JAN 2014
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Journal of Cellular and Molecular Medicine
Volume 18, Issue 4, pages 624–634, April 2014
How to Cite
Chimenti, I., Gaetani, R., Forte, E., Angelini, F., De Falco, E., Zoccai, G. B., Messina, E., Frati, G. and Giacomello, A. (2014), Serum and supplement optimization for EU GMP-compliance in cardiospheres cell culture. Journal of Cellular and Molecular Medicine, 18: 624–634. doi: 10.1111/jcmm.12210
- Issue published online: 8 APR 2014
- Article first published online: 20 JAN 2014
- Manuscript Accepted: 15 NOV 2013
- Manuscript Received: 3 JUL 2013
- “Sapienza” University of Rome. Grant Number: C26A11J528
- Pasteur Institute – Cenci Bolognetti Foundation
Table S1 Sex, age and diagnosis of donor patients.
Table S2 Realtime PCR primers.
Table S3 Statistics of the timing for harvests from primary explant culture in tested AB human sera (HSs) and gamma-irradiated (giFBS) GMP-grade FBSs from different companies.
|jcmm12210-sup-0002-FigS1.tif||image/tif||1482K||Figure S1 Primary explant cultures in 1 or 3% human AB serum.|
|jcmm12210-sup-0003-FigS2.tif||image/tif||5320K||Figure S2 Cell yield, morphology and proliferation of CPCs derived from gamma-irradiated Australian FBSs explant cultures.|
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