Lactoferrin gene knockdown leads to similar effects to iron chelation in human adipocytes
Version of Record online: 26 FEB 2014
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Journal of Cellular and Molecular Medicine
Volume 18, Issue 3, pages 391–395, March 2014
How to Cite
Moreno-Navarrete, J. M., Ortega, F., Moreno, M., Serrano, M., Ricart, W. and Fernández-Real, J. M. (2014), Lactoferrin gene knockdown leads to similar effects to iron chelation in human adipocytes. Journal of Cellular and Molecular Medicine, 18: 391–395. doi: 10.1111/jcmm.12234
- Issue online: 27 FEB 2014
- Version of Record online: 26 FEB 2014
- Manuscript Accepted: 18 DEC 2013
- Manuscript Received: 17 OCT 2013
- Ministerio de Economía y Competitividad. Grant Numbers: PI11/00214, PI12/02631
- iron metabolism
In human and mice adipose tissue, lactoferrin (LTF) has been found to be associated with increased adipogenesis and decreased inflammatory markers. Here, we aimed to investigate the effects of LTF knockdown (KD) in human adipocyte differentiation. In addition, the effects of exogenous LTF administration and iron chelation [using deferoxamine (DFO, 10 μM)] were tested. In both subcutaneous and visceral pre-adipocytes, LTF KD led to decrease significantly adipogenic, lipogenic and insulin signalling-related gene expression and a significant increase in the gene expression of inflammatory mediators. Human lactoferrin (hLf, 1 μM) administration led to recover adipocyte differentiation in LTF KD pre-adipocytes. Interestingly, iron chelation triggered similar effects to LTF KD, decreasing significantly adipogenic gene expressions. Of note, DFO (10 μM) and hLf (1 and 10 μM) co-administration led to a dose-dependent recovery of adipocyte differentiation. These new data reveal that endogenous LTF biosynthesis during human adipocyte differentiation is essential to achieve this process, possibly, modulating adipocyte iron homoeostasis. hLf administration might be a useful therapeutic target in obesity-associated adipose tissue dysfunction.