Expression of dual Nucleotides/Cysteinyl-Leukotrienes Receptor GPR17 in early trafficking of cardiac stromal cells after myocardial infarction
Version of Record online: 7 JUN 2014
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Journal of Cellular and Molecular Medicine
Volume 18, Issue 9, pages 1785–1796, September 2014
How to Cite
Cosentino, S., Castiglioni, L., Colazzo, F., Nobili, E., Tremoli, E., Rosa, P., Abbracchio, M. P., Sironi, L. and Pesce, M. (2014), Expression of dual Nucleotides/Cysteinyl-Leukotrienes Receptor GPR17 in early trafficking of cardiac stromal cells after myocardial infarction. Journal of Cellular and Molecular Medicine, 18: 1785–1796. doi: 10.1111/jcmm.12305
- Issue online: 10 SEP 2014
- Version of Record online: 7 JUN 2014
- Manuscript Accepted: 25 MAR 2014
- Manuscript Received: 8 NOV 2013
- Cariplo Foundation
Figure S1. Left ventricle transversal sections showing the infract region at 24 and 48 hrs after ligation of the coronary artery.
Figure S2. Evaluation of GPR17+ and Sca-1+ cell populations at 48 hrs after MI by immunofluorescence analysis of transversal sections (see Fig. S1 for sections orientation) of the ischaemic LV zones.
Figure S3. Z-stack confocal image of the ischaemic myocardium 24 hrs after ischaemia.
Figure S4. Counting of cells single positive (A) or double/triple positive (B) cells for the indicated markers, after staining with multicolour immunofluorescence and confocal analysis.
Figure S5. GPR17+/CD44+ cells invading the infracted myocardium do not express mature MF marker Collagen-I.
Figure S6. Immunofluorescence showing expression of GPR17 in conjunction with mature myofibroblasts marker αSMA in the ischaemic myocardium at 48 hrs (48h; upper panels) and 1 week (1W; lower panels) after MI.
Figure S7. Phenotypic characterization of Sca-1+ cell line.
Figure S8. Stability of the Sca-1+ cells line and quantification of mesenchymal marker expression.
Data S1. Detailed materials and methods.
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