Characterization of the effect of LPS on dendritic cell subset discrimination in spleen

Authors

  • Kristin L Griffiths,

    1. Research School of Biology, The Australian National University, Canberra, ACT, Australia
    Search for more papers by this author
  • Jonathan KH Tan,

    1. Research School of Biology, The Australian National University, Canberra, ACT, Australia
    Search for more papers by this author
  • Helen C O'Neill

    Corresponding author
    1. Research School of Biology, The Australian National University, Canberra, ACT, Australia
    • Correspondence to: Professor Helen C O'NEILL, Research School of Biology, Bldg 134 Linnaeus Way, The Australian National University, Canberra, ACT 2600, Australia.

      Tel.: +612 6125 4720

      Fax: +612 6125 0313

      E-mail: helen.oneill@anu.edu.au

    Search for more papers by this author

Abstract

The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) is a potent inflammatory mediator and a leading cause of bacterial sepsis. While LPS is known to activate antigen-presenting cells, here we find that LPS down-regulates expression of CD11c and CD11b on splenic dendritic cell subsets, thus confounding the ability to identify these subsets following treatment. This has implications with regard to tracking the response to LPS in terms of the cell subsets involved, and should be considered whenever such studies are undertaken.

Ancillary