Serum supplemented culture medium masks hypertrophic phenotypes in human pluripotent stem cell derived cardiomyocytes
Version of Record online: 1 JUL 2014
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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Journal of Cellular and Molecular Medicine
Volume 18, Issue 8, pages 1509–1518, August 2014
How to Cite
Dambrot, C., Braam, S. R., Tertoolen, L. G. J., Birket, M., Atsma, D. E. and Mummery, C. L. (2014), Serum supplemented culture medium masks hypertrophic phenotypes in human pluripotent stem cell derived cardiomyocytes. Journal of Cellular and Molecular Medicine, 18: 1509–1518. doi: 10.1111/jcmm.12356
- Issue online: 2 SEP 2014
- Version of Record online: 1 JUL 2014
- Manuscript Accepted: 27 MAY 2014
- Manuscript Received: 13 MAR 2014
- The Netherlands Institute for Regenerative Medicine
- The Netherlands Organization for Health Research and Development. Grant Numbers: 114000111, 11.600.1016
Figure S1 Cardiac differentiation efficiency of the Nkx 2-5eGFP/w hESC line and LUMC0004ictrl determined by flow cytometry of cardiac troponin T (cTnT) expression (n = 11 for Nkx 2-5eGFP/w, two independent experiments for LUMC0004ictrl.
Figure S2 Stacked bar graph of the percentage of RAT-CM with sarcomeric structural class (visible well-organized striations (+; category 1), some (disorganized) striations (+/−;category 2), and poor/non-existent striations (−;category 3)); n ≥ 41 cells.
Figure S3 Characterization of hiPSC.
Figure S4 Response of hiPSC-CM to isoproterenol (ISO).
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