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Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo

Authors

  • Sandra Treves-Manusevitz,

    1. Department of Oral Biology, Faculty of Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
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  • Lia Hoz,

    1. Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, México D.F, Mexico
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  • Heled Rachima,

    1. Department of Oral and Maxillofacial Surgery, Sheba Medical Center, Tel Hashomer, Israel
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  • Gonzalo Montoya,

    1. Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, México D.F, Mexico
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  • Ephraim Tzur,

    1. Unit of Maxillofacial Surgery, Assaf Harofeh Medical Center, Zrifin, Israel
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  • Alexander Vardimon,

    1. Unit of Maxillofacial Surgery, Assaf Harofeh Medical Center, Zrifin, Israel
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  • A. Sampath Narayanan,

    1. Department of Orthodontics, Faculty of Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
    2. Department of Pathology, School of Medicine, University of Washington, Seattle, WA, USA
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  • Salomon Amar,

    1. Center for Anti-Inflammatory Therapeutics, School of Dental Medicine, Boston University, Boston, MA, USA
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  • Higinio Arzate,

    Corresponding author
    1. Laboratorio de Biología Periodontal y Tejidos Mineralizados, Facultad de Odontología, Universidad Nacional Autónoma de México, México D.F, Mexico
    • Department of Oral Biology, Faculty of Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
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  • Sandu Pitaru

    Corresponding author
    • Department of Oral Biology, Faculty of Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
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  • Conflict of interest

    Sandu Pitaru is the inventor of a filed patent entitled: “Pluripotent autologous stem cells from oral mucosa and methods of use”.

  • The authors do not have any conflict of interest. This work was supported by the Binational United States – Israel Science Foundation [Grant number 2007049], the Israel Science Foundation of the Israeli Academy of Science [Grant number 646/09] and the Chief Scientist of the Ministry of Science [Grant number191-4201].

Address:

Sandu Pitaru

Department of Oral Biology

Faculty of Medicine

School of Dental Medicine

Tel Aviv University

69978 Tel Aviv

Israel

E-mail: pitaru@post.tau.ac.il

Higinio Arzate

Laboratorio de Biología Periodontal y Tejidos

Mineralizados, Facultad de Odontología

Universidad Nacional Autónoma de México

México D.F.

Mexico

E-mail: harzate@unam.mx

Abstract

Aims

To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva.

Material and Methods

Fibrin–hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed.

Results

Two-week-old cultures of fibrin–hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin–hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates.

Conclusions

Within the limitation of this study, the results indicate for the first time that fibrin–hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.

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