Conflict of interest and sources of funding statement
Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis
Article first published online: 19 OCT 2012
© 2012 John Wiley & Sons A/S
Journal of Clinical Periodontology
Volume 40, Issue 1, pages 18–25, January 2013
How to Cite
Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis. J Clin Periodontol 2012; doi: 10.1111/jcpe.12020, , , , , , .
The authors declare that they have no conflicts of interests. This study population is part of the Health 2000 Health Examination survey, organized by the National Public Health Institute (presently National Institute for Health and Welfare, Finland), and partly supported by the Finnish Dental Association and the Finnish Dental Society Apollonia. Financial support was received from the Finnish Cultural Foundation (for U. K. G), the Sigrid Juselius Foundation (for P. J. P.), the Academy of Finland (grant 78443 for E. K.) and the Helsinki University Central Hospital Research Foundation (for T. S.). Timo Sorsa is an inventor of US-patents 5652227, 5736341, 5866432 and 6143476.
- Issue published online: 11 DEC 2012
- Article first published online: 19 OCT 2012
- Accepted manuscript online: 23 SEP 2012 10:45PM EST
- Manuscript Accepted: 9 SEP 2012
- Manuscript Revised: 29 AUG 2012
- Manuscript Received: 27 JUN 2012
- collagen type I;
Type I collagen degradation end-products and related matrix metalloproteinases (MMPs) were examined aiming to detect potential markers of periodontitis in saliva, with high sensitivity and specificity.
Materials and Methods
The salivary concentrations of MMP-8, MMP-9 and MMP-13, tartrate-resistant acid phosphatase serum type 5b, C-terminal cross-linked telopeptide of type I collagen (CTx), N-terminal cross-linked telopeptide of type I collagen (NTx) and cross-linked carboxyterminal telopeptide of type I collagen were analysed in 230 subjects. Oral health examination included panoramic radiography.
The concentrations of MMP-8, MMP-9 and MMP-13 in saliva were higher in subjects with generalized periodontitis than in controls. Of the tested salivary markers, MMP-8 was the only marker capable of differentiating subjects with severe alveolar bone loss from those with slight bone loss (p < 0.001). The association between the salivary MMP-8 levels and periodontitis remained significant after the adjustment with age, gender and smoking. In addition, significant correlations were found between the tested markers and periodontal parameters.
Enzymes and end-products of type I collagen degradation have different associations with each other and with periodontal status that may reflect their roles in the cascade leading to alveolar bone loss. MMP-8 is a strong biomarker candidate for detecting alveolar bone destruction.