Journal of Clinical Periodontology

Failure to detect an association between aggressive periodontitis and the prevalence of herpesviruses

Authors

  • Jamal M. Stein,

    Corresponding author
    • Department of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital (RWTH), Aachen, Germany
    Search for more papers by this author
    • These authors contributed equally to the study.
  • Sareh Said Yekta,

    1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital (RWTH), Aachen, Germany
    Search for more papers by this author
    • These authors contributed equally to the study.
  • Michael Kleines,

    1. Division of Virology, Department of Hygiene, Microbiology, Social Medicine Innsbruck Medical University, Innsbruck, Austria
    Search for more papers by this author
  • Dilara Ok,

    1. Department of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital (RWTH), Aachen, Germany
    Search for more papers by this author
  • Adrian Kasaj,

    1. Department of Operative Dentistry and Periodontology, University Medical Center, Mainz, Germany
    Search for more papers by this author
  • Stefan Reichert,

    1. Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Martin-Luther University, Halle-Wittenberg, Germany
    Search for more papers by this author
  • Susanne Schulz,

    1. Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Martin-Luther University, Halle-Wittenberg, Germany
    Search for more papers by this author
  • Simone Scheithauer

    1. Department of Infection Control and Infectious Diseases, University Hospital (RWTH), Aachen, Germany
    Search for more papers by this author

  • Conflict of interest and sources of funding statement

    The authors declare that they have no conflict of interests. No external funding, apart from the support of the author's institution, was available for this study.

Address:

Jamal M. Stein

Department of Operative Dentistry

Periodontology and Preventive Dentistry

University Hospital Aachen (RWTH)

Pauwelsstrasse 30, D-52074 Aachen

Germany

E-mail: JStein@ukaachen.de

Abstract

Background

Herpes simplex virus type 1 (HSV-1), human cytomegalovirus (HCMV) and Epstein–Barr virus (EBV) have been suspected to play a causal role in periodontitis pathogenesis. The aim of this study was to determine the prevalence of these viruses in subgingival plaque samples of Caucasian patients with generalized aggressive periodontitis compared to periodontally healthy controls.

Methods

A total of 65 patients with aggressive periodontitis and 65 unmatched controls from Germany were investigated in the study. Subgingival plaque samples were analysed for the presence of HSV-1, EBV and HCMV by quantitative real-time polymerase chain reaction assays. Viral antibody titres were determined quantitatively by immunosorbent assays.

Results

DNA of HSV-1 and HCMV were detected in 1.5% of the patients and controls, whereas EBV DNA was present in 10.8% and 13.9% respectively. Detection rates of serum IgG against HSV-1 (76.1% versus 73.9%), EBV (98.5% versus 96.9%), HCMV (47.7% versus 46.2%) and IgM levels against HSV-1 (6.2% versus 1.5%), EBV (0% versus 0%), HCMV (0% versus 1.5%) did not significantly differ between patients and controls.

Conclusion

The data of our study do not suggest any contribution of HSV-1, EBV or HCMV to aggressive periodontitis in a German population. Ethnic and methodological aspects might have caused conflicting results of previous studies.

Ancillary