Transplantation of embryonic stem cells improves the regeneration of periodontal furcation defects in a porcine model

Authors

  • Jenn-Rong Yang,

    1. Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan
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    • Conflict of interest and source of funding statement

      This study was supported by grant NSC 99-2314-B-006-035-MY3 from the National Science Council, Taiwan. The authors declare that there are no conflicts of interest in this study.

  • Chia-Wen Hsu,

    1. Dental Department, Tainan Municipal Hospital, Tainan, Taiwan
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    • Conflict of interest and source of funding statement

      This study was supported by grant NSC 99-2314-B-006-035-MY3 from the National Science Council, Taiwan. The authors declare that there are no conflicts of interest in this study.

  • Shih-Chung Liao,

    1. Department of Oral Medicine, National Cheng Kung University Hospital, Tainan, Taiwan
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  • Yu-Ting Lin,

    1. Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan
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  • Lih-Ren Chen,

    1. Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan
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  • Kuo Yuan

    Corresponding author
    1. Department of Oral Medicine, National Cheng Kung University Hospital, Tainan, Taiwan
    2. Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    3. School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
    • Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, Taiwan
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  • These authors contributed equally to the article.

†Address:

Kuo Yuan

Institute of Oral Medicine

College of Medicine, National Cheng Kung University

1 University Road, Tainan 701

Taiwan

E-mail: kuoyuan@mail.ncku.edu.tw

Abstract

Objectives

Stem cell-based therapy promises to regenerate lost tissue. Embryonic stem (ES) cells are pluripotent and may provide a virtually unlimited source for transplantation. We investigated whether ES cell transplantation improved the regeneration of furcation defects in a porcine model.

Material and Methods

Experimental periodontitis was induced in the buccal furcations of the bilateral mandibular 2nd premolars of six minipigs. After 4 weeks, the lesions were surgically debrided and implanted with collagen matrix alone (control site) or collagen matrix overlaid with porcine ES cells expressing green fluorescent protein (pES/GFP+) (test site). After 3 months of healing, the clinical parameters were measured again. The treated teeth with adjacent tissue, and part of the major organs, were processed for GFP immunohistochemistry.

Results

We found no obvious teratoma or rejection. The test group had significantly better clinical parameters. Immunohistochemistry (IHC) showed that transplanted pES/GFP+ cells had differentiated to new periodontal ligament and cementum in the test sites. Surprisingly, GFP+ cells were also detectable in the repaired control cementum and remote organs.

Conclusions

We conclude that using ES cells to improve the regeneration of periodontal furcation defects is feasible. More studies are required to assess this potential treatment's efficacy and safety.

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