Rapid identification of Sporothrix schenckii in biopsy tissue by PCR


  • Conflict of interest
    None declared.

  • Funding sources
    This work was supported by National Natural Science Foundation of China (Grant No. 30470104).

  • X. Liu and Z. Zhang contributed equally to this work.


Background  The dimorphic fungus Sporothrix schenckii is the etiological agent of sporotrichosis, an important cutaneous mycosis with a worldwide distribution. At present, it is challenging to rapidly discover and identify Sporothrix schenckii in biopsy tissues nowadays.

Aims  To explore new methods for rapid diagnosis of sporotrichosis.

Materials and Methods  We screened specific primers for Sporothrix schenckii using 50 clinical isolates from patients with sporotrichosis. DNA was extracted from the lesions of 30 cases of clinically suspected sporotrichosis using the Graham s method of CTAB and amplified by PCR using the screened specific primers.

Results  The primer S2-R2 was applicable for the identification of S. schenckii from different geographic areas and clinical types with high specificity and sensitivity. Twenty-five out of the thirty cases (83.3%) amplified using the primer S2-R2 showed positive bands. Further positive bands were observed in 95.6% of cases tested positive by fungal culture.

Conclusions  Using the PCR technique and specific primers, we developed a new diagnostic method that can rapidly diagnose sporotrichosis with tissues obtained from clinical biopsies.