Conflict of interest None declared.
Rapid identification of Sporothrix schenckii in biopsy tissue by PCR
Article first published online: 20 DEC 2012
© 2012 The Authors. Journal of the European Academy of Dermatology and Venereology © 2012 European Academy of Dermatology and Venereology
Journal of the European Academy of Dermatology and Venereology
Volume 27, Issue 12, pages 1491–1497, December 2013
How to Cite
Liu, X., Zhang, Z., Hou, B., Wang, D., Sun, T., Li, F., Wang, H. and Han, S. (2013), Rapid identification of Sporothrix schenckii in biopsy tissue by PCR. Journal of the European Academy of Dermatology and Venereology, 27: 1491–1497. doi: 10.1111/jdv.12030
Funding sources This work was supported by National Natural Science Foundation of China (Grant No. 30470104).
X. Liu and Z. Zhang contributed equally to this work.
- Issue published online: 21 NOV 2013
- Article first published online: 20 DEC 2012
- Received: 15 April 2012; Accepted: 03 October 2012
- National Natural Science Foundation of China. Grant Number: 30470104
Background The dimorphic fungus Sporothrix schenckii is the etiological agent of sporotrichosis, an important cutaneous mycosis with a worldwide distribution. At present, it is challenging to rapidly discover and identify Sporothrix schenckii in biopsy tissues nowadays.
Aims To explore new methods for rapid diagnosis of sporotrichosis.
Materials and Methods We screened specific primers for Sporothrix schenckii using 50 clinical isolates from patients with sporotrichosis. DNA was extracted from the lesions of 30 cases of clinically suspected sporotrichosis using the Graham s method of CTAB and amplified by PCR using the screened specific primers.
Results The primer S2-R2 was applicable for the identification of S. schenckii from different geographic areas and clinical types with high specificity and sensitivity. Twenty-five out of the thirty cases (83.3%) amplified using the primer S2-R2 showed positive bands. Further positive bands were observed in 95.6% of cases tested positive by fungal culture.
Conclusions Using the PCR technique and specific primers, we developed a new diagnostic method that can rapidly diagnose sporotrichosis with tissues obtained from clinical biopsies.