jdv12086-sup-0001-FigureS1.docxWord document97KFigure S1. Cultured fibroblasts were treated with tacrolimus 0, 1, 10, 100 and 1000 nmol/L and incubated for 48 h. Total cellular protein lysates were extracted and subjected to Western blotting analysis. GAPDH expression was served as internal control. The results showed that various concentrations of tacrolimus treatment did not significantly affect type I collagen expression in normal human fibroblasts. The plots shown here were from two different experiments with similar results.

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