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Eosinophil cationic protein levels parallel coagulation activation in the blister fluid of patients with bullous pemphigoid

Authors

  • A. Tedeschi,

    Corresponding author
    1. Unità Operativa di Allergologia e Immunologia Clinica, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano, Italy
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  • A.V. Marzano,

    1. Unità Operativa di Dermatologia, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano, Italy
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  • M. Lorini,

    1. Unità Operativa di Allergologia e Immunologia Clinica, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano, Italy
    2. Dipartimento di Fisiopatologia medico-chirurgica e dei trapianti, Università degli studi di Milano, Milano, Italy
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  • Y. Balice,

    1. Unità Operativa di Dermatologia, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano, Italy
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  • M. Cugno

    1. Dipartimento di Fisiopatologia medico-chirurgica e dei trapianti, Università degli studi di Milano, Milano, Italy
    2. Unità Operativa di Medicina Interna 2, Ospedale Maggiore Policlinico, Fondazione IRCCS Ca' Granda, Milano, Italy
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  • Conflicts of interest

    None declared.

  • Funding sources

    This work was partially supported by ‘Ricerca corrente 2012', Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano.

Abstract

Background

Bullous pemphigoid (BP) is an autoimmune blistering disease due to autoantibodies against two hemidesmosomal antigens, namely BP180 and BP230, and characterized by coagulation activation both at cutaneous and systemic levels. Skin-infiltrating eosinophils contribute to bulla formation and, upon activation, are supposed to initiate the coagulation cascade.

Objective

The aim of this study was to investigate whether the activation of eosinophils and coagulation are linked in BP.

Methods

We evaluated the correlation between eosinophil cationic protein (ECP) levels and concentrations of the prothrombotic markers F1 + 2 and D-dimer in blister fluid and blood samples of 30 BP patients. Thirty healthy subjects were used as normal controls.

Results

ECP, F1 + 2 and D-dimer plasma levels were significantly higher in BP patients than in normal subjects. A significant correlation was found between ECP plasma levels and blood eosinophil count (r = 0.54, P = 0.002). F1 + 2 plasma levels positively correlated with disease severity, expressed as the percentage of body surface area involved (r = 0.36, P = 0.048). A striking increase in ECP (288.8 ± 45.2 ng/mL), F1 + 2 (31 409.9 ± 2929.4 pmol/L) and D-dimer levels (342 798.3 ± 44 206 ng/mL) was found in blister fluid from BP patients. In blister fluid, ECP levels were significantly higher than in peripheral blood (P < 0.0001) and were positively correlated with the levels of both F1 + 2 (r = 0.4, P = 0.02) and D-dimer (r = 0.5, P = 0.0045).

Conclusions

ECP levels are strikingly elevated in blister fluids from BP patients and correlate with markers of coagulation activation, supporting the view that eosinophils initiate the coagulation cascade at skin level.

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