Efficacy of antifungal PACT in an in vitro model of onychomycosis

Authors


  • Conflicts of interest

      Conflicts of interest
    • F. Schynowski is an employee of Cumdente GmbH and developed the LED lamp. R. Hahn is the owner of Hahn Medical Systems GmbH. All other authors report no conflicts of interest.
  • Funding sources

      Funding sources
    • The experiments have been financially supported by Cumdente GmbH, Germany. Also, Cumdente GmbH provided indirect funding by providing the LED and the photoactivating gel.

Abstract

Background

The difficulty of antifungal substances to penetrate keratin and slow nail growth limit the efficacy of topical therapy in onychomycosis. One promising alternative is photodynamic antimicrobial chemotherapy, or PACT: an irradiated photosensitizer creates singlet oxygen molecules which destroy pathogens without damaging human cells.

Objective

As PACT has demonstrated strong antifungal capabilities, we wanted to investigate its efficacy in an in vitro model of onychomycosis.

Methods

PACT was tested in a microdilution assay, in an in vitro onychomycosis model as well as in a patient.

Results

PACT inhibited fungal growth in the microdilution assay with no colonies of T. rubrum detectable. Fungal growth was also inhibited in an onychomycosis model, after 30 min of LED irradiation. Subsequently, a patient with distolateral onychomycosis was treated on three consecutive days and showed significant and durable improvement of nail morphology 6 months after.

Conclusion

PACT appears to be an effective treatment of onychomycosis in vitro. The promising results need to be validated by clinical trials.

 

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