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Table S1 Table of all site/location data.

Table S2 Body shape anova table.

Table S3 Foraging and anti-predator trait anova tables.

Table S4 Macroinvertebrate isotope anova.

Table S5 Stickleback global GLMMs and local GLMs.

Table S6 Global GLMMs and local GLMs on estimated proportions of end member sources to diet.

Table S7 mancova test for parallelism using all samples (invariant and variant rivers).

Table S8 Results for deviation from Hardy–Weingberg Equilibrium for whole catchment and by river location (using Bonferroni corrections and False Discovery Rate).

Figure S1 Deformation grids showing shape differences in female and male individuals along PC1; note the change in snout length.

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