Fold or hold: experimental evolution in vitro

Authors

  • S. Collins,

    Corresponding author
    1. Ashworth Laboratories, Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, UK
    • Correspondence: Sinead Collins, Ashworth Laboratories, Institute of Evolutionary Biology, University of Edinburgh, The King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK.

      Tel.: +44 (0) 131 650 8657; fax: +44 (0)131 650 5455; e-mail: s.collins@ed.ac.uk

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  • A. Rambaut,

    1. Ashworth Laboratories, Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, UK
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  • S. J. Bridgett

    1. Ashworth Laboratories, Institute of Evolutionary Biology, The Gene Pool Genomics Facility, University of Edinburgh, Edinburgh, UK
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Abstract

We introduce a system for experimental evolution consisting of populations of short oligonucleotides (Oli populations) evolving in a modified quantitative polymerase chain reaction (qPCR). It is tractable at the genetic, genomic, phenotypic and fitness levels. The Oli system uses DNA hairpins designed to form structures that self-prime under defined conditions. Selection acts on the phenotype of self-priming, after which differences in fitness are amplified and quantified using qPCR. We outline the methodological and bioinformatics tools for the Oli system here and demonstrate that it can be used as a conventional experimental evolution model system by test-driving it in an experiment investigating adaptive evolution under different rates of environmental change.

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