Real-time PCR for identification of the soybean aphid, Aphis glycines Matsumura
Article first published online: 10 FEB 2014
© 2014 The Authors Journal of Applied Entomology Published by Blackwell Verlag GmbH.
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Journal of Applied Entomology
Volume 138, Issue 7, pages 485–489, August 2014
How to Cite
Naaum, A. M., Foottit, R. G., Maw, H. E. L. and Hanner, R. (2014), Real-time PCR for identification of the soybean aphid, Aphis glycines Matsumura. Journal of Applied Entomology, 138: 485–489. doi: 10.1111/jen.12114
- Issue published online: 9 JUL 2014
- Article first published online: 10 FEB 2014
- Manuscript Accepted: 27 NOV 2013
- Manuscript Received: 8 AUG 2013
- Ontario Ministry of Agriculture Food and Rural Affairs (OMAFRA)
- Emergency Management Research Program
- DNA barcoding;
- non-destructive DNA extraction;
- pest identification;
- real-time PCR;
- soybean aphid
The soybean aphid (Aphis glycines Matsumura) is an economically significant pest in North America, causing extensive damage to soybean crops through direct feeding damage and disease transmission. If unchecked, this pest could cause billions of dollars of damage to soybean crops. Identification of the soybean aphid can be difficult due to its small size, complex life cycle and morphological plasticity. Generally, an expert is required to identify a specimen. Additionally, identification of some life stages, such as eggs, is impossible. DNA barcoding has been successfully used to differentiate aphid species, including A. glycines, based on sequencing of a standardized gene region. Although this method represents an important step towards accurate identification, samples must still be sent to specialized facilities for analysis. Using existing DNA barcode sequences in the publically accessible Barcode of Life Data System (BOLD; www.boldsystems.org), species-specific differences were identified and used to develop a real-time PCR assay to identify soybean aphids. This assay can be run on portable systems for rapid, accurate and simple identification in the field. The use of a non-destructive DNA extraction protocol allows the original insect to be vouchered and therefore available for further study if necessary. This work represents an important step in soybean aphid management.