Get access

The Multilayered Interlaced Network (MIN) in the sporoplasm of the Microsporidium Anncaliia algerae is derived from Golgi

Authors

  • Peter M. Takvorian,

    Corresponding author
    1. Department of Pathology, Division of Tropical Medicine and Parasitology, Albert Einstein College of Medicine, Bronx, New York
    • Department of Biological Sciences, Rutgers University, Newark, New Jersey
    Search for more papers by this author
  • Karolyn F. Buttle,

    1. New York State Dept of Health, Resource for Visualization of Biological Complexity, Wadsworth Center, Albany, New York
    Search for more papers by this author
    • This study is dedicated to the memory of Karolyn Buttle, an inspiring colleague and friend.
  • David Mankus,

    1. New York State Dept of Health, Resource for Visualization of Biological Complexity, Wadsworth Center, Albany, New York
    Search for more papers by this author
  • Carmen A. Mannella,

    1. New York State Dept of Health, Resource for Visualization of Biological Complexity, Wadsworth Center, Albany, New York
    Search for more papers by this author
  • Louis M. Weiss,

    1. Department of Pathology, Division of Tropical Medicine and Parasitology, Albert Einstein College of Medicine, Bronx, New York
    2. Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, New York
    Search for more papers by this author
  • Ann Cali

    1. Department of Biological Sciences, Rutgers University, Newark, New Jersey
    Search for more papers by this author

Correspondence

P. Takvorian, Department of Biological Sciences, 195 university Avenue, Rutgers University, Newark, New Jersey 07102, USA

Telephone number: 973 353 5364; FAX number: 973-353-5518 e-mail: petetak@andromeda.rutgers.edu

Abstract

This study provides evidence for the Golgi-like activity of the multilayered interlaced network (MIN) and new ultrastructural observations of the MIN in the sporoplasm of Anncaliia algerae, a microsporidium that infects both insects and humans. The MIN is attached to the end of the polar tubule upon extrusion from the germinating spore. It surrounds the sporoplasm, immediately below its plasma membrane, and most likely maintains the integrity of the sporoplasm, as it is pulled through the everting polar tube. Furthermore, the MIN appears to deposit its dense contents on the surface of the sporoplasm within minutes of spore discharge thickening the plasma membrane. This thickening is characteristic of the developmental stages of the genus Anncaliia. The current study utilizes transmission electron microscopy (TEM), enzyme histochemistry, and high voltage TEM (HVEM) with 3D tomographic reconstruction to both visualize the structure of the MIN and demonstrate that the MIN is a Golgi-related structure. The presence of developmentally regulated Golgi in the Microsporidia has been previously documented. The current study extends our understanding of the microsporidial Golgi and is consistent with the MIN being involved in the extracellular secretion in Anncaliia algerae. This report further illustrates the unique morphology of the MIN as illustrated by HVEM using 3D tomography.

Get access to the full text of this article

Ancillary