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jeu12019-sup-0001-FigS1.pdfapplication/PDF579KFig. S1. A–D Enzyme histochemical controls A, B are images of thin sections of host cell cultures (RK-13) exposed to spores of Anncaliia algerae for 1 h. Post exposure (PE) the cells were briefly immuno-fixed (glutaraldehyde and paraformaldehyde), incubated in complete IDPase reaction media with sucrose substituted for the substrate IDP, then processed for TEM and stained with only uranyl acetate. The lack of deposition of electron-dense reaction product in both the host cell and the sporoplasm demonstrates the specificity of the cis-Golgi associated enzyme and serves as a control for both the host cell and sporoplasm MIN. A - Bar = 1 μm. B - Bar = 500 nm. C, D are images of thin sections of host cell cultures (RK-13) exposed to spores of Anncaliia algerae for 1 h. Post exposure (PE) the cells were briefly immuno-fixed(glutaraldehyde and paraformaldehyde), incubated in complete TPPase reaction media with sucrose substituted for the substrate STP, then processed for TEM and stained with only uranyl acetate. The lack of deposition of electron-dense reaction product in both the host cell and the sporoplasm demonstrates the specificity of the trans-Golgi associated enzyme and serves as a control for both the host cell and sporoplasm MIN. C – Bar = 1 μm. D – Bar = 200 nm.
jeu12019-sup-0002-MovieS1.movvideo/mov2077KMovie S1. A “walk through” movie of the 100 images used for the tomographically reconstructed sporoplasm volume.
jeu12019-sup-0003-MovieS2.movvideo/mov13858KMovie S2. A “walk through” movie of a 400-nm –thick portion of the sporoplasm tomogram after denoising by anisotrophic diffusion, followed by a 3D model (generated by masking and isodensity surface rendering). This “walk through” illustrates the sporoplasm ultrastructure, its plasma membrane continuity, and relationship with this segment of the MIN. The cut-away 3D model overlay of the MIN (yellow) and sporoplasm plasma membrane (green) indicate the intimate interconnections between them. This portion of the polar tube (red) on the sporoplasm surface provides a good reference point for the viewer.
jeu12019-sup-0004-MovieS3.movvideo/mov3898KMovie S3. A movie showing rotation of a 3D model of the MIN region, generated by denoising (as in Movie S2) masking (to remove the plasma membrane, cytoplasm, and polar tube) and isodensity surface rendering. This model depicts portions of fenestrated flattened sheets, numerous saccular and tubular structures, and anastomosing tubules that are part of the MIN's complex structure. Typical Golgi (COP-I & II) vesicles and buds are not present, further indicating the similarity of this 3D model to that of the Golgi of the microsporidium Paranosema.

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