A New Freshwater Amoeba: Cochliopodium pentatrifurcatum n. sp. (Amoebozoa, Amorphea)



Y.I. Tekle, Department of Biology, Spelman College, 350 Spelman Lane Southwest, Atlanta, Georgia 30314

Telephone number: +1 404-270-5779;

FAX number: +1 404-270-5725

e-mail: ytekle@spelman.edu


Cochliopodium pentatrifurcatum n. sp. (ATCC© 30935TM) is described based on light microscopic morphology, fine structure, and molecular genetic evidence. Cochliopodium pentatrifurcatum n. sp. (length ~ 25 μm) is characterized by surface microscales (0.3 μm tall) containing a circular porous base (~ 0.6 μm diam.) with a thin peripheral rim. Five radially arranged feet, emanating from the base, support a short central column terminating apically as a funnel-shaped collar (~ 0.5 μm diam.) composed of five radial, trifurcate rays extending from the center toward a thin peripheral rim. The central spine is 0.5–0.6 μm long. The comparative morphologies and combined molecular genetic evidence, SSU-rDNA and COI, indicate that the new species falls in a clade sufficiently different from other species to suggest that it is a valid new species.