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Fig. S1. Sediment temperature profiles below the surface of Beggiatoa mats.

Fig. S2. Cluster analysis of Sørensen similarities for ciliate and bacterial communities.

Table S1. Sequence identity and relevant environmental information for ciliate sequence data included in phylogenetic analysis.

Table S2. Trace metal concentrations (μM) of water samples collected above each mat. Numbers below elements refer to atomic weights.

Table S3. Most abundant sequences and their distribution across mat environments. Numbers indicate the number of sequences in duplicate cores from each environment. Closest environmental and cultured ciliate matches are indicated for each OTU along with % similarity and GenBank accession numbers.

Table S4. Distribution of Novel Deep-Sea clade of ciliates across mat environments at Guaymas. Numbers indicate the number of sequences for the OTU in duplicate cores from each environment.

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