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Fig. S1–S11. Micrographs of specimens sequenced. S1, S2. Codonellopsis gaussi (T95 and T96). S3, S4. Laackmanniella prolongata (T97 and T98). S5. Amphorellopsis quinquealata (T108). S6. Cymatocylis drygalskii (T101). S7–S9. Cymatocylis calyciformis (T105, T99 and T106). S10, S11. Cymatocylis convallaria (T107 and T104). The micrographs of T100 (Cymatocylis calyciformis), T103 (Codonellopsis gaussi) and T102 (Amphorellopsis quinquealata) are excluded due to low resolution. All images were taken without a coverglass because of the next step (DNA extraction), and were distorted by water.

Fig. S12–S18. Original image of Fig. 27–33. Micrographs of Codonellopsis gaussi after protargol impregnation. S12. Ventral view showing buccal cavity. S13. Lateral view showing lateral and left ciliary field. S14. Dorsal view showing dorsal kinety, left and right ciliary field. S15. Right ciliary field. S16. Dorsal view showing dorsal kinety. S17. Macronuclei. Ventral view. Arrow head marks the micronuclei. S18. Dorsal view showing dorsal and posterior kinety. BM = buccal membranelles; DK = dorsal kinety; F = fibers; LA = lateral ciliary field; LF = left ciliary field; Ma = macronuclei; PK = posterior kinety; RF = right ciliary field.

Fig. S19–S24. Original image of Fig. 34–39. Micrographs of Laackmanniella prolongata after protargol impregnation. S19. Dorsal view showing dorsal kinety. S20. Ventral view showing ventral kinety, lateral and left ciliary field. S21. Macronuclei and micronuclei. S22. Ventral view of oral primordium. S23. Dorsal view of dorsal kinety and right ciliary filed. S24. Ventral view showing posterior kinety. DK = dorsal kinety; F = fibers; LA = lateral ciliary field; LF = left ciliary field; Ma = macronuclei; Mi = micronuclei; PK = posterior kinety; RF = right ciliary field; VK = ventral kinety.

Table S1. Primers used for DNA sequencing.

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