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Fig. S1. Effect of melatonin on gene expression regulated by auxin in Arabidopsis thaliana primary root meristems. GUS staining of primary roots of DR5:GUS A. thaliana seedlings treated for 7 d with (A) the solvent; and in liquid medium supplemented with (B) 10 μM IAA, (C) 5 μM NAA, or (D–F) Melatonin at 50, 100, and 500 μM, respectively. Note the failure of melatonin to activate GUS expression. Photographs are representative of at least 15 stained seedlings. Scale bars: 50 μM (A–F).

Fig. S2. Effect of melatonin on gene expression regulated by auxin in Arabidopsis thaliana lateral root primordia. Short time GUS staining of roots of DR5:GUS A. thaliana seedlings treated for 7 d with (A) the solvent; and in liquid medium supplemented with (B) 10 μM IAA, (C) 5 μM NAA, or (DF) Melatonin at 50, 100, and 500 μM, respectively. Note the failure of melatonin to activate GUS expression. Photographs are representative of at least 15 stained seedlings. Scale bars: 50 μM (A–F).

Table S1. Genes analyzed by real time PCR after treatment with N-acetyl serotonin or IAA. The relative expression variation should be higher than twofold and therefore the mean of relative expression (MRE) in log2 > 1. SD, standard deviation; N, number of isolated experiments.

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