jfb12031-sup-0001-TableS1.docWord document38K Table SI. Components and cycling reaction for the PCR of the three mitochondrial genes, control region, cyt b and nd2. Concentration of each PCR component is indicated. The cycle number, time (min) and temperature (° C) are indicated for each step of the cycling reaction. All cycling reactions involved an initial denaturation at 94° C for 3 min for cyt b and nd2 and for 2 min for the control region, and a final extension step at 72° C for 10 min. The final volume of the reaction was 30 µl and contains 50–200 ng of DNA. Taq DNA polymerase, PCR buffer, MgCl2 and deoxynucleotide triphosphates were from Invitrogen
jfb12031-sup-0002-TableS2.docWord document88K Table SII. GenBank accession numbers of the sequences used in the phylogenetic analysis of Chilean Orestias
jfb12031-sup-0003-TableS3.docWord document71K Table SIII. GenBank accession numbers of the haplotypes used in the phylogeographic analysis of Orestias ascotanensis

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