Neuropeptide Y in black seabream Acanthopagrus schlegelii: identification, distribution and mRNA expression responses to ghrelin

Authors

  • X. L. Ma,

    1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, PR China
    2. Department of Life Science, Huizhou University, Huizhou, PR China
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  • L. W. Zheng,

    1. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, PR China
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  • L. T. Mao,

    1. Department of Life Science, Huizhou University, Huizhou, PR China
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  • L. B. Zhou,

    1. Department of Life Science, Huizhou University, Huizhou, PR China
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  • A. L. Wang

    Corresponding author
    1. Department of Life Science, Huizhou University, Huizhou, PR China
    • Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou, PR China
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Author to whom correspondence should be addressed. Tel.: +86 20 85210141; email: wanganl1957@163.com

Abstract

The coding region of neuropeptide Y (NPY) complementary (c)DNA was cloned from the hypothalamus RNA of black seabream Acanthopagrus schlegelii, including 297 bp coding for prepro-NPY of 98 amino acids. Real-time reverse transcriptase-polymerase chain reaction was performed to determine A. schlegelii npy gene expression; NPY messenger RNA (mRNA) was expressed highly in the brain and stomach. Treatment with acylated ghrelin significantly up-regulated NPY mRNA level in the hypothalamus, suggesting that NPY may be involved in regulating food intake of A. schlegelii.

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