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Keywords:

  • genetics;
  • ichthyoplankton;
  • Istiophorus platypterus;
  • minimally invasive;
  • NaOH;
  • Thunnus albacares

A rapid non-destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor.