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Figure S1 Western blot analysis of PAK2 siRNA-transfected HepG2 cells. After 24 h of transfection, cells were serum-deprived for 24 h and treated with TGF-β for 2 h. Columns show relative band intensities expressed as the fold changes relative to control cells. Data are expressed as mean ± SD of three independent experiments (NS, not significant).

Figure S2 Western blot analysis of PAK2 siRNA-transfected HepG2 cells. Twenty-four hours after transfection, cells were serum-deprived for 24 h before being treated with TGF-β for 2 h with or without a 1 h pretreatment with either LY294002 or NAC. Columns show relative band intensities expressed as the fold changes compared to control cells. Data are expressed as mean ± SD of three independent experiments (**P < 0.01 vs non-treated cells: NS, not significant).

Figure S3 Cell migration assay of PAK2 siRNA-treated cells. Twenty-four hours after transfection, cells were treated with TGF-β for an additional 24 h with or without pretreatment with LY294002 or NAC for 1 h. The cells were suspended in DMEM containing 1% FBS and plated at 1×104 cells/mL in the upper chambers of transwell supports with 8-mm pores (BD Biosciences). The lower chambers were filled with medium containing 10% FBS. After 10 h, the cells that had migrated through the pores were counted in five random microscopic fields, and the average number of migrated cells in control siRNA-transfected wells was set at 100%. Data are expressed as mean ± SD of three independent experiments (**P < 0.01 vs non-treated cells: NS, not significant).

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