Effects of splenectomy on hepatic gene expression profiles after massive hepatectomy in rats
Article first published online: 23 SEP 2013
© 2013 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd
Journal of Gastroenterology and Hepatology
Volume 28, Issue 10, pages 1669–1677, October 2013
How to Cite
Arakawa, Y., Shimada, M., Utsunomya, T., Imura, S., Morine, Y., Ikemoto, T. and Takasu, C. (2013), Effects of splenectomy on hepatic gene expression profiles after massive hepatectomy in rats. Journal of Gastroenterology and Hepatology, 28: 1669–1677. doi: 10.1111/jgh.12316
- Issue published online: 23 SEP 2013
- Article first published online: 23 SEP 2013
- Accepted manuscript online: 28 JUN 2013 05:54AM EST
- Manuscript Accepted: 21 MAY 2013
- early growth response 1;
- heat shock protein;
- heme oxygenase-1;
- liver regeneration;
- reperfusion injury;
Background and Aim
Possible spleno-hepatic relationships affected by hepatectomy still remained unclear. We have previously reported that splenectomy may ameliorate liver injuries and promote appropriate liver regeneration after massive hepatectomy. Therefore, we investigated the effects of splenectomy on the DNA expression profile in the liver after massive hepatectomy in rats.
Rats were divided into the following two groups: 90% hepatectomy (Hx group) and 90% hepatectomy with splenectomy (Hx + Sp group). Rats were sacrificed 3 and 6 h after surgery, and mRNA from liver tissue was isolated and hybridized to Affymetrix GeneChip Rat Genome 230 2.0 Array (Affymetrix, Santa Clara, CA, USA) and a pathway analysis was done with Ingenuity Pathway Analysis (Ingenuity Systems, Mountain View, CA, USA).
We determined the Hx + Sp/Hx ratio to assess the influence of splenectomy, and cut-off values were set at more than 2.0-fold or less than 1/2 (0.5)-fold. Immediate early response gene including early growth response-1 and FBJ murine osteosarcoma-related pathways were markedly downregulated by splenectomy. In contrast, heme oxygenase-1 gene-related pathway was upregulated by splenectomy.
Splenectomy provided the protective effects for liver failure and promoted liver regeneration, possibly owing to the downregulation of immediate early response genes and upregulation of the heat shock protein, heme oxygenase-1.